Re use of sequencing consumables after pre run check failures

This article provides guidance on when consumables can be reused if encountering pre-run check failures or failures right after the run starts, depending on the Illumina platform.

iSeq 100

Pre-run check failures:

The pre-run checks consist of a dry stage followed by a fluidics stage. The consumables cannot be reused after the transition from the dry check to the fluidics check. This transition occurs at approximately 33% of the pre-run checks progress.

• Flow check errors: With the error message "The flow check failed, and consumables cannot be reused. Power cycle the instrument per system guide instructions and retry the run.", the consumables have been marked as used, and cannot be reused.

MiSeq

Pre-run check failures:

Consumables can be reused on the same or another instrument.

Failures after run start:

• SIPDOWN errors: Consumables can be reused if error happens before the start of the run on the same or another instrument if the reagent cartridge has not been pierced. For more information, see Troubleshooting SIPDOWN Errors on the MiSeq. Contact Illumina Technical Support for further assistance.

MiniSeq and NextSeq 500/550

Pre-run check failures:

If a pre-run check fails, the reagent cartridge integrated RFID (radio-frequency identification) is not locked and can be used for a subsequent run.

• Failures requiring a power cycle: Pre-run check failures may require a power cycle to resolve. For NextSeq 500/550 Control Software versions older than v4.2, the consumables must be removed from the instrument before power cycling. Failure to remove consumables prior to power cycling results in consumables marked as used. For NextSeq Control Software v4.2 and later, the consumables are not marked as used when instrument is power cycled. For more information, see Best practices for NextSeq 500/550 pre-run check failures requiring a power cycle.

Failures after starting the run:

• Failed to communicate with RTA: When this error occurs before the reagent cartridge is pierced, the reagent cartridge can be reused for a new run setup. For more information, see Troubleshooting "Failed to communicate with RTA after 4 attempts" on the MiniSeq or NextSeq 500/550.

NextSeq 1000/2000

Pre-run check failures:

• Failures with alignment check: If this error occurs during the dry pre-run checks, the consumables are not marked as used. Consumables can be reused on the same or different instrument, if the error is transient and unrelated to the flow cell. See Pre-Run check failed with an alignment check failure on the NextSeq 1000/2000.

• Failures related to temperature: Consumables can potentially be reused. Contact Illumina Technical Support for further assistance.

• Failures at the fluidics stage: Consumables cannot be reused. For more information, see Pre-run check failed at Fluidics stage on the NextSeq 1000/2000.

MiSeq i100

Pre-run check failures:

The consumables can be reused if the pre-run checks fail before they reach approximately 25%. After approximately 25%, the consumables are pierced and cannot be reused.

NovaSeq 6000

Pre-run check failures:

NovaSeq 6000 pre-run check errors do not lock the consumable RFIDs (Radio-frequency identification). Therefore, the consumables can be reused.

Failures after start of run:

• "A component failed during sequencing” error within first 10 minutes after starting the run: If runs on the NovaSeq 6000 exit within the first 10 minutes after start with a "A component failed" error message, confirm that the reagents are unpierced, and no run folder has been generated under C:\ProgramData*\Illumina\NovaSeq\NovaSeqTemp. The reagents may be reused in a follow up sequencing run. For more information, see How to troubleshoot “A component failed during sequencing” error messages on the NovaSeq 6000. Contact Illumina Technical Support for further assistance.

NovaSeq X Series

Pre-run check failures:

• Software check and dry check failures: Consumables can potentially be reused.

• Wet check failures: Flow cell and buffer cartridge cannot be reused. The lyo insert, Library Tube Strip, and reagent cartridge are not pierced and can be recovered.

Note: The reagent doors can still be opened if a run is being performed on the other side of the instrument.

For any feedback or questions regarding this article (Illumina Knowledge Article #9623), contact Illumina Technical Support [e-mail].