How does the Illumina/Nextera tagmentation enzyme make sure that both p5 and p7 adapters are added?

The tagmentation enzyme used by Illumina is pre-loaded with both read 1 and read 2 DNA oligo tags in a DNA-enzyme complex which makes sure that sequences are added to both ends of the DNA fragmentation sites. This is the tagmentation strategy which is used in both bead-linked and free-solution tagmentation enzymes. Subsequent protocol steps are optimized to produce library fragments with one p5 side adapter and one p7 side adapter to allow for sequencing compatibility.

The following kits use a free-solution transposome

• Nextera XT

• Illumina Tagment DNA Enzyme and Buffer Kits

  

The following kits use a bead-linked transposome (note that the following use different bead-linked transposome versions)

• Illumina DNA Prep

• Illumina DNA Prep with Enrichment

• Illumina DNA PCR-Free

• Illumina RNA Prep with Enrichment (tagments double stranded cDNA)

  

The bead-linked transposomes allows for greater flexibly of input DNA amounts so that a wide range of DNA inputs can be used to achieve a consistent library size.

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