Compatible and recommended Illumina library types for the NovaSeq 6000 sequencing platform

Illumina offers a wide variety of library preparation kits and sequencing platforms for different applications, each with its own coverage and indexing considerations. The table below lists the sequencing primers included in the NovaSeq 6000 reagent cartridges, and the Illumina library preparation kits compatible with the NovaSeq platform based on the required data output and the indexing capabilities of each kit.

Note: The information in the tables below is based on the required data output and indexing capabilities of each kit. Most Illumina libraries can be sequenced with all Illumina sequencing platforms. If a specific Illumina library is not listed below, contact Illumina Tech Support to discuss special considerations.

Refer to the Sequence Coverage Calculator and Library Prep and Array Kit Selector for additional information.

Table 1. Sequencing primer compatibility on the NovaSeq platform with NovaSeq v1 reagents, based on recommended library and flow cell types.

Table 2. Sequencing primer compatibility on the NovaSeq platform with NovaSeq v1.5 reagents, based on recommended library and flow cell types.

For instructions on how to use custom primers on the NovaSeq sequencing platform, refer to the following resources:

• NovaSeq Series Custom Primers Guide

• Spiking custom primers into the Illumina sequencing primers

For any feedback or questions regarding this article (Illumina Knowledge Article #1541), contact Illumina Technical Support [e-mail].