# FAQ

Library Preparation > Illumina Single Cell RNA Prep > FAQ

* [Can vortexing damage cells/nuclei when generating emulsions in the Illumina Single Cell workflow?](https://knowledge.illumina.com/library-preparation/illumina-single-cell-rna-prep/library-preparation-illumina-single-cell-rna-prep-faq-list/000009458)
* [How to determine the required sequencing read depth for Illumina Single Cell Prep?](https://knowledge.illumina.com/library-preparation/illumina-single-cell-rna-prep/library-preparation-illumina-single-cell-rna-prep-faq-list/000009561)
* [Illumina Single Cell 3' RNA demonstrated cell and sample types](https://knowledge.illumina.com/library-preparation/illumina-single-cell-rna-prep/library-preparation-illumina-single-cell-rna-prep-faq-list/000009642)
* [Validated plasticware for PIPs handling in Illumina Single Cell 3’ RNA Prep](https://knowledge.illumina.com/library-preparation/illumina-single-cell-rna-prep/library-preparation-illumina-single-cell-rna-prep-faq-list/000009621)
* [What is the maximum cell size that can be captured in Illumina Single Cell kits?](https://knowledge.illumina.com/library-preparation/illumina-single-cell-rna-prep/library-preparation-illumina-single-cell-rna-prep-faq-list/000009828)