# FAQ

Library Preparation > Illumina Single Cell RNA Prep > FAQ

* [Can vortexing damage cells/nuclei when generating emulsions in the Illumina Single Cell workflow?](/library-preparation/illumina-single-cell-rna-prep/library-preparation-illumina-single-cell-rna-prep-faq-list/000009458.md)
* [How to determine the required sequencing read depth for Illumina Single Cell Prep?](/library-preparation/illumina-single-cell-rna-prep/library-preparation-illumina-single-cell-rna-prep-faq-list/000009561.md)
* [Illumina Single Cell 3' RNA demonstrated cell and sample types](/library-preparation/illumina-single-cell-rna-prep/library-preparation-illumina-single-cell-rna-prep-faq-list/000009642.md)
* [Validated plasticware for PIPs handling in Illumina Single Cell 3’ RNA Prep](/library-preparation/illumina-single-cell-rna-prep/library-preparation-illumina-single-cell-rna-prep-faq-list/000009621.md)
* [What is the maximum cell size that can be captured in Illumina Single Cell kits?](/library-preparation/illumina-single-cell-rna-prep/library-preparation-illumina-single-cell-rna-prep-faq-list/000009828.md)


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