Chemistry and imaging on MiSeq
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Four-Channel Sequencing by Synthesis (SBS) Chemistry
In four-channel SBS, bases are identified using four different fluorophores for each base and four images per sequencing cycle.
The sequencing cycle begins with a chemistry step where all four deferentially labeled bases are added to the flow cell.
Following nucleotide incorporation, the imaging cycle begins and includes the capture of four distinct images using four different wavelength bands.
The images are processed with image analysis software to determine which nucleotides were incorporated at each cluster position across the flow cell.
With four-channel sequencing, every sequencing cycle requires four dyes and four images to determine the DNA sequence.
The MiSeq and HiSeq Series Systems currently use four-channel SBS.
For more information, see SBS Technology Spotlight.
For any feedback or questions regarding this article (Illumina Knowledge Article #3757), contact Illumina Technical Support techsupport@illumina.com.
