System Checks for the NextSeq 1000/2000

A system check is not required for normal operation or instrument maintenance. Illumina Technical Support may request the system checks for troubleshooting pre-run check errors or other issues. The NextSeq 1000/2000 system check is comprised of four subsystem checks and takes about 58 minutes to complete. The tests confirm whether components are properly aligned and functional. Check results are output to the system-check folder located in /usr/local/illumina/systemcheck.

Unload the cartridge before running system checks. Failure to remove the cartridge prior to starting a system check can damage to the system.

Running a System Check

1. From the control software menu, select System Checks.

2. Select the checkbox for any of the following system checks to be performed.

• Network Connectivity:Checks the network connection status and performance.

• Enclosure:Checks the performance of the thermal system and visor lift mechanism.

• Motion:Checks the travel limits and performance of the Z-stage and XY-stage.

• Optics:Checks the performance of the imaging module.

1. Select Start.

Example:

This information is also available in video form. Search for Knowledge Base article How to perform a System Check on the NextSeq 1000/2000 Video.

Instructions for performing a system check on the NextSeq 1000/2000 are in the Troubleshooting Section of the NextSeq 1000/2000 Sequencing System Guide.

For any feedback or questions regarding this article (Illumina Knowledge Article #3457), contact Illumina Technical Support [e-mail].