> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/ampliseq.md).

# AmpliSeq

- [FAQ](https://knowledge.illumina.com/library-preparation/ampliseq/library-preparation-ampliseq-faq-list.md)
- [Recommended positive control for AmpliSeq for Illumina BRCA Panel library prep](https://knowledge.illumina.com/library-preparation/ampliseq/library-preparation-ampliseq-faq-list/000005659.md)
- [Recommended positive controls for AmpliSeq for Illumina Cancer Hotspot v2 Panel library prep](https://knowledge.illumina.com/library-preparation/ampliseq/library-preparation-ampliseq-faq-list/000005660.md)
- [What is the difference between AmpliSeq for Illumina custom DNA HotSpot and Gene Design Panels?](https://knowledge.illumina.com/library-preparation/ampliseq/library-preparation-ampliseq-faq-list/000001052.md)
- [Which files can be obtained from DesignStudio for custom AmpliSeq sequencing panels?](https://knowledge.illumina.com/library-preparation/ampliseq/library-preparation-ampliseq-faq-list/000005717.md)
- [Reference Material](https://knowledge.illumina.com/library-preparation/ampliseq/library-preparation-ampliseq-reference_material-list.md)
- [Number of libraries per AmpliSeq for Illumina Library PLUS kit for custom and community panels](https://knowledge.illumina.com/library-preparation/ampliseq/library-preparation-ampliseq-reference_material-list/000002098.md)
- [Number of reactions per AmpliSeq for Illumina custom spike in panel order](https://knowledge.illumina.com/library-preparation/ampliseq/library-preparation-ampliseq-reference_material-list/000001239.md)
- [Number of tubes in an AmpliSeq Custom or Community Panel orders](https://knowledge.illumina.com/library-preparation/ampliseq/library-preparation-ampliseq-reference_material-list/000001234.md)
- [Performing a spike in of custom oligos into an AmpliSeq for Illumina Panel](https://knowledge.illumina.com/library-preparation/ampliseq/library-preparation-ampliseq-reference_material-list/000001238.md)
- [Plate layout for AmpliSeq Unique Dual (UD) indexes](https://knowledge.illumina.com/library-preparation/ampliseq/library-preparation-ampliseq-reference_material-list/000002334.md)
- [Plate layouts for Ampliseq Combinatorial Dual (CD) Indexes Sets A, B, C and D](https://knowledge.illumina.com/library-preparation/ampliseq/library-preparation-ampliseq-reference_material-list/000002333.md)


---

# Agent Instructions
This documentation is published with GitBook. GitBook is the documentation platform designed so that both humans and AI agents can read, navigate, and reason over technical content effectively. Learn more at gitbook.com.

## Querying This Documentation
If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter, and the optional `goal` query parameter:

```
GET https://knowledge.illumina.com/library-preparation/ampliseq.md?ask=<question>&goal=<endgoal>
```

`ask` is the immediate question: it should be specific, self-contained, and written in natural language.
`goal` is optional and describes the broader end goal you are ultimately trying to accomplish on behalf of the user. GitBook uses it to tailor the answer towards what is most useful for that goal.

The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.