# Illumina非Pattern Flow Cell测序平台簇密度推荐指南

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簇密度是决定测序中能否获得最佳的测序质量和数据量的重要因素。下表是根据平衡文库（例如phix 文库）的结果，列出的各平台原始簇密度推荐值：

| 测序平台            | 模式/试剂                      | 原始簇密度理想值                   |
| --------------- | -------------------------- | -------------------------- |
| MiSeq           | v2                         | 1000-1200 K/mm<sup>2</sup> |
| v3              | 1200-1400 K/mm<sup>2</sup> |                            |
| MiniSeq         | Mid and High Output        | 170-220 K/mm<sup>2</sup>   |
| NextSeq 500/550 | Mid and High Output        | 170-220 K/mm<sup>2</sup>   |

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其中iSeq 100,  MiSeq i100系列, NextSeq 1000/2000, NovaSeq 6000, NovaSeq X 系列测序平台使用的Flow Cell为Pattern Flow Cell, 这种类型的Flow Cell即使是上面所有纳米孔都没有成簇，簇密度也都为同一固定值。在使用这些平台时可以通过Cluster PF(%)对Cluster Occupancy做出初步的判断。

详情请参阅以下资料:

* [通过绘制% Occupied和% Pass Filter散点图，优化NovaSeq 6000和iSeq 100测序平台的上样浓度](https://knowledge.illumina.com/instrumentation/general/instrumentation-general-reference_material-list/000007138)
* [Calculating Percent Passing filter for Patterned and Nonpatterned Flow Cells](https://www.illumina.com/content/dam/illumina-marketing/documents/products/technotes/hiseq-x-percent-pf-technical-note-770-2014-043.pdf)

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