> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/microarray/general/microarray-general-faq-list/000005874.md).

# What is the correct order of addition of Infinium Amplification reagents?

The Infinium assay workflow includes a critical DNA amplification step. Before amplification, genomic DNA must be combined with MA1 and NaOH to facilitate denaturation and stabilization of the reaction. MA1 plays a key role in preventing evaporation of both NaOH and DNA during incubation and thus,must always be added first, regardless of assay type.

Recommended order of adding amplification reagents:

* Infinium HD and HTS Assays: MA1 > **DNA > NaOH** > MA2 > MSM
* Infinium HD Methylation and LCG Assays: MA1 > **DNA > NaOH** > RPM > MSM
* Infinium XT Assay : MA1 > **NaOH > DNA** >MA2 > RAM
* Infinium EX Assay: MA1 > **NaOH > DNA >** MA2 > AAX
* Infinium EX Methylation Assay: MA1 > **NaOH > DNA** >MPM > MSM

These reagent addition sequences have been validated by Illumina. Adhering to the correct order of reagent addition ensures consistent and high-quality assay performance. Illumina does not guarantee assay performance if the protocol is not followed as specified as deviations may negatively impact results.

**Refer to the Infinium Product Documentation for more information:**

* [Infinium HD Assay Ultra Protocol Guide](https://support.illumina.com/downloads/infinium_hd_ultra_assay_protocol_guide_\(11328087_b\).html)
* [Infinium HD Super Assay Reference Guide](https://support.illumina.com/downloads/infinium-hd-super-assay-reference-guide-11322427.html)
* [Infinium HD Methylation Assay Product Documentation](https://support.illumina.com/downloads/infinium-hd-methylation-reference-guide-15019519.html)
* [Infinium HTS Assay Reference Guide](https://support.illumina.com/downloads/infinium-hts-assay-reference-guide-15045738.html)
* [**Infinium HTS Extra Reference Guide**](https://support.illumina.com/downloads/infinium-hts-extra-assay-reference-guide-1000000041072.html)
* [Infinium LCG Assay Product Documentation](https://support.illumina.com/downloads/infinium-lcg-assay-reference-guide-15023139.html)
* [**Infinium LCG Quad Assay Protocol Guide**](https://support.illumina.com//downloads/infinium_lcg_quad_assay_guide_15025908_b.html)
* Infinium XT Assay Reference Guide: [ST Workflow](https://support.illumina.com/downloads/infinium-xt-st-reference-guide-1000000025687.html) | [HT Workflow](https://support.illumina.com/downloads/infinium-xt-ht-reference-guide-1000000016440.html)
* [Infinium EX Assay Product Documentation](https://support-docs.illumina.com/ARR/Inf-EX/Content/ARR/FrontPages/inf_ex_assay.htm)
* [Infinium EX Methylation Assay Product Documentation](https://support-docs.illumina.com/ARR/Inf-MSA/Content/ARR/FrontPages/inf_ex_methylation_assay.htm)

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #5874), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000005874%20-%20Microarray%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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