What is the correct order of addition of Infinium Amplification reagents?

The purpose of MA1 is to prevent evaporation of NaOH and DNA in the plate.

• MA1 must always be added first

• Order of addition for legacy Infinium protocols (HD, HTS, LCG): MA1 > DNA > NaOH

• Order of addition for newer Infinium protocols (XT, EX): MA1 > NaOH > DNA

These conditions have been validated by Illumina. Assay performance is not guaranteed if there are deviations from the protocol.

Refer to the Infinium Product Documentation for more information:

• Infinium HD Assay Ultra Protocol Guide

• Infinium HD Super Assay Reference Guide

• Infinium HD Methylation Assay Product Documentation

• Infinium HTS Assay Reference Guide

• Infinium LCG Assay Product Documentation

• Infinium XT Assay Reference Guide: ST Workflow | HT Workflow

• Infinium EX Assay Product Documentation

• Infinium EX Methylation Assay Product Documentation

For any feedback or questions regarding this article (Illumina Knowledge Article #5874), contact Illumina Technical Support [e-mail].