# What is the correct order of addition of Infinium Amplification reagents?

The purpose of MA1 is to prevent evaporation of NaOH and DNA in the plate.

* MA1 must always be added first
* Order of addition for legacy Infinium protocols (HD, HTS, LCG): MA1 > DNA > NaOH
* Order of addition for newer Infinium protocols (XT, EX): MA1 > NaOH > DNA

These conditions have been validated by Illumina. Assay performance is not guaranteed if there are deviations from the protocol.

**Refer to the Infinium Product Documentation for more information:**

* [Infinium HD Assay Ultra Protocol Guide](https://support.illumina.com/downloads/infinium_hd_ultra_assay_protocol_guide_\(11328087_b\).html)
* [Infinium HD Super Assay Reference Guide](https://support.illumina.com/downloads/infinium-hd-super-assay-reference-guide-11322427.html)
* [Infinium HD Methylation Assay Product Documentation](https://support.illumina.com/downloads/infinium-hd-methylation-reference-guide-15019519.html)
* [Infinium HTS Assay Reference Guide](https://support.illumina.com/downloads/infinium-hts-assay-reference-guide-15045738.html)
* [Infinium LCG Assay Product Documentation](https://support.illumina.com/downloads/infinium-lcg-assay-reference-guide-15023139.html)
* Infinium XT Assay Reference Guide: [ST Workflow](https://support.illumina.com/downloads/infinium-xt-st-reference-guide-1000000025687.html) | [HT Workflow](https://support.illumina.com/downloads/infinium-xt-ht-reference-guide-1000000016440.html)
* [Infinium EX Assay Product Documentation](https://support-docs.illumina.com/ARR/Inf-EX/Content/ARR/FrontPages/inf_ex_assay.htm)
* [Infinium EX Methylation Assay Product Documentation](https://support-docs.illumina.com/ARR/Inf-MSA/Content/ARR/FrontPages/inf_ex_methylation_assay.htm)

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #5874), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000005874%20-%20Microarray%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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