Library loading concentration considerations for the iSeq 100

On the iSeq 100 the loading volume for all library types is 20 µl.

Library type	v2 Loading Concentration (pM)	v1 Loading Concentration* (pM)
100% PhiX	100	60
AmpliSeq for Illumina Library PLUS	50	50
Illumina DNA Prep	100	200
Illumina DNA Prep with Enrichment	75	100
Nextera XT DNA	150	50
TruSeq DNA Nano	150	100
TruSeq DNA PCR-Free	100	100
Illumina DNA PCR-Free	120	N/A

For other library types, 50 pM is the recommended starting loading concentration.

Important note: The above recommendations are general starting points. Optimize concentrations over subsequent runs to identify a loading concentration that consistently yields data that meets run specifications.

This information can be found in the iSeq 100 System Guide.

\*v1 reagents for the iSeq 100 have been discontinued, but the information for them remains here to assist labs still finishing their v1 inventory.

For any feedback or questions regarding this article (Illumina Knowledge Article #2121), contact Illumina Technical Support [e-mail].