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Library loading concentration considerations for the iSeq 100

On the iSeq 100 the loading volume for all library types is 20 µl.
Library type
v2 Loading Concentration (pM)
v1 Loading Concentration* (pM)
100% PhiX
100
60
AmpliSeq for Illumina Library PLUS
50
50
Illumina DNA Prep
100
200
Illumina DNA Prep with Enrichment
75
100
Nextera XT DNA
150
50
TruSeq DNA Nano
150
100
TruSeq DNA PCR-Free
100
100
Illumina DNA PCR-Free
120
N/A
For other library types, 50 pM is the recommended starting loading concentration.
Important note: The above recommendations are general starting points. Optimize concentrations over subsequent runs to identify a loading concentration that consistently yields data that meets run specifications.
This information can be found in the iSeq 100 System Guide.
\*v1 reagents for the iSeq 100 have been discontinued, but the information for them remains here to assist labs still finishing their v1 inventory.
For any feedback or questions regarding this article (Illumina Knowledge Article #2121), contact Illumina Technical Support [email protected].
Last modified 24d ago
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