Troubleshooting Pressure Charging Failures on the MiSeq i100 Series

Background

The MiSeq i100 Series uses pressure assisted fluidics to move reagents through the cartridge and flow cell. This requires that the wet cartridge is pressurized to a specific pressure range, and must be maintained throughout the sequencing run.

If there are issues with the reagent seals on the wet cartridge, this can cause issues with the Pre-Run Checks or during the instrument sequencing runs. If this occurs during a sequencing run, the Control Software presents the following error:

The pressure charging failed due to air leakage detected. Contact Illumina Technical Support.

If this issue is observed, see the following Troubleshooting Actions and contact Illumina Tech Support.

Troubleshooting Actions

1. Select Eject Cartridge, then remove the dry and wet sequencing cartridges from the instrument.

2. Power cycle the instrument to ensure all board communications are reset.

   1. See Knowledge Article How to Power Cycle the MiSeq i100 Series for more information.
3. Run the instrument Mechanical and Fluidics System Health Checks using the Test Cartridges provided with the instrument.

   1. See Knowledge Article How to Setup the System Health Checks on the MiSeq i100 Series Instruments for more information.
4. Once the System Health Checks are complete, select Clear Checks and then proceed to the Control Software home screen.

5. Use the Export Logs - Small option to bundle the System Health Check results.

   1. If applicable, select the checkbox next to any affected Sequencing Run(s) from the list to include the run Logs folder.

   2. See Knowledge Article How to Export Logs on the MiSeq i100 Series for more information.
6. Once the Export Logs workflow has completed and transferred to the External Storage, contact Illumina Tech Support for further steps.

For any feedback or questions regarding this article (Illumina Knowledge Article #9266), contact Illumina Technical Support [e-mail].