# FAQ

Instrumentation > NextSeq 1000/2000 > FAQ

* [Can NextSeq 1000/2000 custom primers be diluted in in RSB with Tween instead of HT1?](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000006981)
* [Can NextSeq 1000/2000 run folders be set up to be deleted automatically?](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000005385)
* [Can a NextSeq 1000/2000 reagent cartridge be used if the foil packaging has been pierced?](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000008942)
* [Can a NextSeq 1000/2000 sequencing run be set up with fewer than 26 cycles in read 1?](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000006385)
* [Can a run be started on the NextSeq 1000/2000 without an active DRAGEN license?](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000003224)
* [Can a run on the NextSeq 1000/2000 use expired reagents?](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000003822)
* [Can additional users be added to the Linux interface on the NextSeq 1000/2000?](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000003802)
* [Can the NextSeq 1000/2000 run on 120V or 240V power?](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000005326)
* [Common Questions Related to Sequencing Analysis Viewer (SAV) software and the NextSeq 1000/2000](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000007863)
* [Custom recipes for Illumina Stranded libraries on NextSeq 1000/2000](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000005583)
* [Does the NextSeq 1000/2000 have thumbnail images available for troubleshooting?](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000003096)
* [Does the NextSeq 1000/2000 require a wash after run completes?](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000005500)
* [How to create new user accounts on the NextSeq 1000/2000](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000006467)
* [How to dispose of a NextSeq 1000/2000 flow cell?](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000006133)
* [How to obtain a NextSeq 1000/2000 v2 sample sheet?](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000003093)
* [How to switch user accounts on NextSeq 1000/2000](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000007856)
* [Is there a maximum number of cycles for index reads on the NextSeq 1000/2000?](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000003459)
* [What is the difference between RSB with Tween 20, part number 20047304 and 20050639?](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000006829)
* [What is the difference between process management and disk management on the NextSeq 1000/2000?](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000005707)
* [Which NextSeq 1000/2000 run modes require a v2 sample sheet?](https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000003092)