# FAQ

Instrumentation > NextSeq 1000/2000 > FAQ

* [Can NextSeq 1000/2000 custom primers be diluted in in RSB with Tween instead of HT1?](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000006981.md)
* [Can NextSeq 1000/2000 run folders be set up to be deleted automatically?](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000005385.md)
* [Can a NextSeq 1000/2000 reagent cartridge be used if the foil packaging has been pierced?](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000008942.md)
* [Can a NextSeq 1000/2000 sequencing run be set up with fewer than 26 cycles in read 1?](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000006385.md)
* [Can a run be started on the NextSeq 1000/2000 without an active DRAGEN license?](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000003224.md)
* [Can a run on the NextSeq 1000/2000 use expired reagents?](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000003822.md)
* [Can additional users be added to the Linux interface on the NextSeq 1000/2000?](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000003802.md)
* [Can the NextSeq 1000/2000 run on 120V or 240V power?](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000005326.md)
* [Common Questions Related to Sequencing Analysis Viewer (SAV) software and the NextSeq 1000/2000](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000007863.md)
* [Custom recipes for Illumina Stranded libraries on NextSeq 1000/2000](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000005583.md)
* [Does the NextSeq 1000/2000 have thumbnail images available for troubleshooting?](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000003096.md)
* [Does the NextSeq 1000/2000 require a wash after run completes?](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000005500.md)
* [How to create new user accounts on the NextSeq 1000/2000](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000006467.md)
* [How to dispose of a NextSeq 1000/2000 flow cell?](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000006133.md)
* [How to obtain a NextSeq 1000/2000 v2 sample sheet?](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000003093.md)
* [How to switch user accounts on NextSeq 1000/2000](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000007856.md)
* [Is there a maximum number of cycles for index reads on the NextSeq 1000/2000?](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000003459.md)
* [What is the difference between RSB with Tween 20, part number 20047304 and 20050639?](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000006829.md)
* [What is the difference between process management and disk management on the NextSeq 1000/2000?](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000005707.md)
* [Which NextSeq 1000/2000 run modes require a v2 sample sheet?](/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list/000003092.md)


---

# Agent Instructions: Querying This Documentation

If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter:

```
GET https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-faq-list.md?ask=<question>
```

The question should be specific, self-contained, and written in natural language.
The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.
