Returning NextSeq 1000/2000 reagents to storage

Use the following instructions to store a thawed NextSeq 1000/2000 reagent cartridge and flow cell in the event of an instrument error during the instrument pre-run check prior to the fluidics check.

1. Separate the flow cell from the cartridge.

2. Remove and discard diluted library from the reservoir (up to ~18 µl).

• Prepare a fresh dilution of the same library for the next run to avoid sample cross-contamination with residual library in the reservoir.

3. Position the cartridge in 2°C to 8°C storage so that the label faces up and air can circulate on all sides. Do not exceed 72 hours of storage at 2°C to 8°C. If a 50-cycle, 100-cycle, 200-cycle, or 300-cycle cartridge was thawed in the refrigerator for 12 hours overnight, do not exceed 60 additional hours. If 600-cycle cartridge was thawed in the refrigerator for 16 hours overnight, do not exceed 56 additional hours.

4. Return the flow cell to the original silver foil packaging with the desiccant.

5. Tape the foil packaging to seal it and place in 2°C to 8°C storage.

• Do not exceed 72 hours.

See NextSeq 1000/2000 Product Documentation Return Consumables to Storage for more information.

For any feedback or questions regarding this article (Illumina Knowledge Article #3033), contact Illumina Technical Support [e-mail].