# Returning NextSeq 1000/2000 reagents to storage

Use the following instructions to store a thawed NextSeq 1000/2000 reagent cartridge and flow cell in the event of an instrument error during the instrument pre-run check **prior to the fluidics check**.

1. Separate the flow cell from the cartridge.
2. Remove and discard diluted library from the reservoir (up to \~18 µl).

* Prepare a fresh dilution of the same library for the next run to avoid sample cross-contamination with residual library in the reservoir.

3. Position the cartridge in 2°C to 8°C storage so that the label faces up and air can circulate on all sides.

* * XLEAP-SBS consumables:\
    \- If cartridge was thawed in the refrigerator for 16 hours overnight, do not exceed 56 hours for all kit configurations.
  * Standard SBS:\
    \- Do not exceed 72 hours of storage at 2°C to 8°C.\
    \- If a 50-cycle, 100-cycle, 200-cycle, or 300-cycle cartridge was thawed in the refrigerator for 12 hours overnight, do not exceed 60 additional hours.\
    \- If 600-cycle cartridge was thawed in the refrigerator for 16 hours overnight, do not exceed 56 additional hours.

4. Return the flow cell to the original silver foil packaging with the desiccant.
5. Tape the foil packaging to seal it and place in 2°C to 8°C storage.

See [NextSeq 1000/2000 Product Documentation Return Consumables to Storage](https://support-docs.illumina.com/IN/NextSeq10002000/Content/IN/NextSeq2000_1000/ReturnConsumablesStorage_fNS.htm) for more information.

\
\
\ <br>

|                                                                                                                                                                                                                                                                                                                                                                        |
| :--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #3033), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000003033%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


---

# Agent Instructions: Querying This Documentation

If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter:

```
GET https://knowledge.illumina.com/instrumentation/nextseq-1000-2000/instrumentation-nextseq-1000-2000-reference_material-list/000003033.md?ask=<question>
```

The question should be specific, self-contained, and written in natural language.
The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.