# Troubleshooting Possible Stale Image Data Detected on the NextSeq 500/550

The following warning occurs when issues arise during flow cell scanning in sequencing and can arise in imaging a single tile. It is usually reflected in poor sequencing metrics, such as overclustering, low passing filter, or a steep intensity/Q30 decline but does not cause the run to stop.

***Warning Occurred: Possible stale image data detected***\
\&#xNAN;***Warning Occurred: Saturated image data detected***\
![](/files/G37wlflXpKtTmEMrSmIA)

**Troubleshooting steps:**

1. Evaluate run performance.
2. Complete automatic post-run wash.
3. Perform system checks.
4. Share results with [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/).

For reference, search Knowledge Base articles:

* **How to perform washes on the NextSeq 500/550**
* **How to perform a NextSeq 500/550 system check**

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #1388), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000001388%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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