# Troubleshooting Possible Stale Image Data Detected on the NextSeq 500/550 The following warning occurs when issues arise during flow cell scanning in sequencing and can arise in imaging a single tile. It is usually reflected in poor sequencing metrics, such as overclustering, low passing filter, or a steep intensity/Q30 decline but does not cause the run to stop. ***Warning Occurred: Possible stale image data detected***\ \&#xNAN;***Warning Occurred: Saturated image data detected***\ ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-d43165d38e5824c61fd26d2fb0eb12284d174022%2Fimage1.png?alt=media\&token=4d120c31-fc7c-4c51-8dc6-d802b191c829) **Troubleshooting steps:** 1. Evaluate run performance. 2. Complete automatic post-run wash. 3. Perform system checks. 4. Share results with [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/). For reference, search Knowledge Base articles: * **How to perform washes on the NextSeq 500/550** * **How to perform a NextSeq 500/550 system check** \ \ \
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