# Illumina RNA Prep Tagmentation (L) with Enrichment General FAQs What **is the Illumina RNA Prep Tagmentation (L) with Enrichment kit?** The Illumina RNA Prep, Tagmentation (L) with Enrichment kit generates targeted RNA libraries that are compatible with Illumina NGS platforms. The workflow is enrichment-based, meaning it uses oligo probes to pull-down regions of interest for sequencing. **Why use a targeted RNA workflow instead of total RNA or mRNA sequencing?** A targeted approach allows for sequencing a specific set of RNA transcripts. This reduces the number of required reads per sample and allows higher sequencing throughput. **Which reagents are needed to order to complete the workflow?** The reference guide lists the required Illumina kits and user-supplied consumables that are required for this application. **Which RNA molecules are targeted with this kit?** The kit selectively captures the regions for which the capture probes have been designed to enrich. **What RNA molecules are depleted with this kit?** There are no rRNA depletion or mRNA capture steps at the beginning of the library prep. Unwanted RNAs are not targeted for enrichment. **Are the libraries stranded?** The libraries are not stranded. \ \ \
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