Illumina RNA Prep Tagmentation (L) with Enrichment General FAQs

What is the Illumina RNA Prep Tagmentation (L) with Enrichment kit?

The Illumina RNA Prep, Tagmentation (L) with Enrichment kit generates targeted RNA libraries that are compatible with Illumina NGS platforms. The workflow is enrichment-based, meaning it uses oligo probes to pull-down regions of interest for sequencing.

Why use a targeted RNA workflow instead of total RNA or mRNA sequencing?

A targeted approach allows for sequencing a specific set of RNA transcripts. This reduces the number of required reads per sample and allows higher sequencing throughput.

Which reagents are needed to order to complete the workflow?

The reference guide lists the required Illumina kits and user-supplied consumables that are required for this application.

Which RNA molecules are targeted with this kit?

The kit selectively captures the regions for which the capture probes have been designed to enrich.

What RNA molecules are depleted with this kit?

There are no rRNA depletion or mRNA capture steps at the beginning of the library prep. Unwanted RNAs are not targeted for enrichment.

Are the libraries stranded?

The libraries are not stranded.

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