# Illumina RNA Prep Tagmentation (L) with Enrichment General FAQs

What **is the Illumina RNA Prep Tagmentation (L) with Enrichment kit?**

The Illumina RNA Prep, Tagmentation (L) with Enrichment kit generates targeted RNA libraries that are compatible with Illumina NGS platforms. The workflow is enrichment-based, meaning it uses oligo probes to pull-down regions of interest for sequencing.

**Why use a targeted RNA workflow instead of total RNA or mRNA sequencing?**

A targeted approach allows for sequencing a specific set of RNA transcripts. This reduces the number of required reads per sample and allows higher sequencing throughput.

**Which reagents are needed to order to complete the workflow?**

The reference guide lists the required Illumina kits and user-supplied consumables that are required for this application.

**Which RNA molecules are targeted with this kit?**

The kit selectively captures the regions for which the capture probes have been designed to enrich.

**What RNA molecules are depleted with this kit?**

There are no rRNA depletion or mRNA capture steps at the beginning of the library prep. Unwanted RNAs are not targeted for enrichment.

**Are the libraries stranded?**

The libraries are not stranded.

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