Sequencing primer compatibility and recommended library types for the HiSeq X and HiSeq 3000/4000

Illumina offers a wide array of library preparation kits and sequencing platforms for different applications, each with its own coverage and indexing considerations. Table 1. and Table 2. list the sequencing primers included in the HiSeq X and HiSeq 3000/4000 reagent kits, and include the Illumina library preparation kits compatible with the HiSeq X and HiSeq 3000/4000 platforms based on the required data output and the indexing capabilities of each kit.

Note: The information in the tables below is based on the required data output and indexing capabilities of each kit. Most Illumina libraries can be sequenced with all Illumina sequencing platforms. If a specific Illumina library is not listed below, contact Illumina Tech Support to discuss special considerations.

Refer to the Sequence Coverage Calculator and Library Prep and Array Kit Selector resources for additional information.

Table 1. Sequencing primer compatibility with Illumina library prep kits recommended for the HiSeq X sequencing platform.

Table 2. Sequencing primer compatibility with Illumina library prep kits recommended for the HiSeq 3000/4000 sequencing platform.

For instructions to use custom primers on the HiSeq 3000/4000 sequencing platforms, refer to the following resources:

• Spiking custom primers into the Illumina sequencing primers

• HiSeq System Custom Primers Guide

For any feedback or questions regarding this article (Illumina Knowledge Article #1503), contact Illumina Technical Support [e-mail].