# Failure to find fiducial errors on the NextSeq 1000/2000
**Background**: If the system is unable to detect clusters in the first cycle of imaging, the run may exit with the following error:
"A failure occurred during sequencing, and consumables cannot be reused. Before setting up a new run, power cycle the instrument per system guide instructions. If the error persists, contact Illumina Technical Support."
When seen at cycle 1, approximately 4 hours after run start, the most common root cause is a "Failure to find fiducial" error.
The NextSeq 1000/2000 completes an autocenter process in the blue channel at cycle 1 to find initial focus. With Patterned Flow Cell Technology, the NextSeq 1000/2000 uses clustered fiducials for Autocenter. Successful Autocenter images require signal present in blue channel at cycle 1.
**Troubleshooting**
Library Considerations
* Use provided RSB+Tween 20 to dilute library to 2nM and then to loading concentration
* Confirm library doesn't have a T overcall at cycle 1
* If running Illumina mRNA stranded library with T over call at cycle 1, a custom recipe is required\
\- NextSeq 1000/2000 control software version 1.2 and earlier, use the custom recipe found at [NextSeq 1000/2000 Sequencing System Product Files](https://support.illumina.com/sequencing/sequencing_instruments/nextseq-1000-2000/product-files.html)\
\- NextSeq 1000/2000 control software version 1.3 and later, custom recipe is integrated into instrument cloud run setup
Run Setup
* Confirm protocol for loading library matches what is chosen in runparameters.xml
* loaded double stranded DNA and false is listed
* manual denature and dilute and true is listed
* If using custom primers, confirm they are indicated in runparameters.xml
Reagent Preparation
* Reagent thawed and prepared in accordance with recommendations in [NextSeq 1000/2000 Sequencing System Guide](https://support-docs.illumina.com/IN/NextSeq10002000/Content/IN/NextSeq2000_1000/ThawBaggedCartridge_fNS.htm)
After reviewing library, run setup, and reagent preparation, contact Illumina Technical Support for further troubleshooting and provide the following:
1. Collect run cycle logs located at /usr/local/Illumina/runs/\[runfolder]
2. Collect the AutoCenter Images at /usr/local/Illumina/runs/\[runfolder]/Autocenter
3. Contact [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/united-states/technical-support) for a file transfer link and further troubleshooting
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| *For any feedback or questions regarding this article (Illumina Knowledge Article #3549), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000003549%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |
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