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Troubleshooting NovaSeq 6000 failing to initialize control software at 94 95%

When a NovaSeq 6000 fails to initialize the Control Software at around 94-95%, collect the following files:

  • NovaSeq Control Software Logs: C:\ProgramData*\Illumina\NovaSeq\NovaSeq Control Software\Logs (zipped)

  • NovaSeq Service Software Logs: C:\ProgramData*****\Illumina\NovaSeq\NovaSeq Service Software\Logs

*The ProgramData folder may be hidden; to un-hide files, select the View tab in the file explorer window and make sure “Hidden items” is checked.

After collecting the logs, contact Illumina Technical Supportarrow-up-right to determine the next steps.

For any feedback or questions regarding this article (Illumina Knowledge Article #1277), contact Illumina Technical Support [email protected]envelope.

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