Resources for iSeq 100 Control Software v3.0

iSeq 100 Control Software v3.0 was launched globally on 15-NOV-2021. The release included software resolutions for both run time estimate inaccuracies and issues during Windows 10 operating system updates, as well as an updated 300-cycle recipe to resolve low-occupancy runs. Most notably, the release of version 3.0 enables the use of custom primers with the iSeq 100 for the first time. See the resources below for assistance in utilizing, navigating, and troubleshooting the iSeq 100 Control Software v3.0.

iSeq 100 Support Resources Home Page

• Support documentation

• Software Downloads

• Product Information

• Tools

• Training Videos (iSeq 100: How to Start a Run now contains section 3.3 outlining the custom primers workflow)

Online iSeq 100 System Guide (English and All Available Language Versions)

Relevant Knowledge Base articles (search each title for more information):

• Sequencing Primer Compatibility for the iSeq 100 Sequencing Platform

• Spiking Custom Primers into the Illumina Sequencing Primers

• Library Denaturation and Compatibility for the iSeq 100 and NextSeq 2000 Systems

• Local Run Manager 2: How to use a Custom Library Prep Kit

• Custom Primer Requirements for the Illumina DNA PCR-Free Prep, Tagmentation Kit

For the iSeq 100 Control Software v3.0 installer, contact Illumina Technical Support ([e-mail]).

For any feedback or questions regarding this article (Illumina Knowledge Article #6125), contact Illumina Technical Support [e-mail].