Which NextSeq 1000/2000 run modes require a v2 sample sheet?

Local mode is the only run mode that requires a v2 sample sheet to start a sequencing run. A sample sheet can optionally be applied during standalone mode run setup if the run is to be sent to BaseSpace Sequence Hub for secondary analysis.

Cloud run and Hybrid run modes are planned via BaseSpace Instrument Run Setup and do not require a v2 sample sheet to start the run.

For any feedback or questions regarding this article (Illumina Knowledge Article #3092), contact Illumina Technical Support [e-mail].