Input types FAQ for Illumina DNA PCR Free libraries

Are there any known inhibitors of the enzymes or reagents in the Illumina PCR-Free kit that should be avoided in the input material?

• See the DNA Input Recommendations section of the Reference Guide for input requirements.

• See the Nextera XT Library Prep: Tips and Troubleshooting guide for information on other contaminants that can interfere with the enzyme in Illumina DNA PCR-Free Prep, Tagmentation kit.

Can large amplicons be used as input for targeted sequencing? This has not been tested.

Does the Illumina PCR-Free kit work on single-stranded DNA input? No, double-stranded DNA input is required.

Can RNA be used as input? No, Illumina DNA PCR-Free only works with double-stranded DNA input.

Does the Illumina PCR-Free kit work on environmental DNA samples (Soil, water, etc.)? This has not been tested.

Can buccal swabs be used for the Illumina PCR-Free kit? This has not been tested.

Can non-human gDNA input be used, such as bacterial DNA?

Yes. See application note Microbial whole-genome sequencing with Illumina DNA PCR-Free Prep, Tagmentation.

For any feedback or questions regarding this article (Illumina Knowledge Article #3438), contact Illumina Technical Support [e-mail].