Input types FAQ for Illumina DNA PCR Free libraries
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Are there any known inhibitors of the enzymes or reagents in the Illumina PCR-Free kit that should be avoided in the input material?
See the DNA Input Recommendations section of the Reference Guide for input requirements.
See the Nextera XT Library Prep: Tips and Troubleshooting guide for information on other contaminants that can interfere with the enzyme in Illumina DNA PCR-Free Prep, Tagmentation kit.
Can large amplicons be used as input for targeted sequencing? This has not been tested.
Does the Illumina PCR-Free kit work on single-stranded DNA input? No, double-stranded DNA input is required.
Can RNA be used as input? No, Illumina DNA PCR-Free only works with double-stranded DNA input.
Does the Illumina PCR-Free kit work on environmental DNA samples (Soil, water, etc.)? This has not been tested.
Can buccal swabs be used for the Illumina PCR-Free kit? This has not been tested.
Can non-human gDNA input be used, such as bacterial DNA?
Yes. See application note Microbial whole-genome sequencing with Illumina DNA PCR-Free Prep, Tagmentation.
For any feedback or questions regarding this article (Illumina Knowledge Article #3438), contact Illumina Technical Support .