> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000006540.md).

# What is the minimum library size that can be sequenced?

**What is the minimum library size that can be sequenced?**

If a fragment has complete adapter sequences, it can bind and cluster. The shortest Illumina library type is TruSeq small RNA libraries, which have \~20 bp inserts. Further, adapter dimers, with no inserts but with the complete adapter sequences, are about 120-150 bp and can cluster and sequence.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #6540), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000006540%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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