What is the minimum library size that can be sequenced?
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What is the minimum library size that can be sequenced?
If a fragment has complete adapter sequences, it can bind and cluster. The shortest Illumina library type is TruSeq small RNA libraries, which have ~20 bp inserts. Further, adapter dimers, with no inserts but with the complete adapter sequences, are about 120-150 bp and can cluster and sequence.
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