What is the minimum library size that can be sequenced?

What is the minimum library size that can be sequenced?

If a fragment has complete adapter sequences, it can bind and cluster. The shortest Illumina library type is TruSeq small RNA libraries, which have ~20 bp inserts. Further, adapter dimers, with no inserts but with the complete adapter sequences, are about 120-150 bp and can cluster and sequence.

For any feedback or questions regarding this article (Illumina Knowledge Article #6540), contact Illumina Technical Support [e-mail].