Illumina Single Cell 3' RNA demonstrated cell and sample types
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Illumina Single Cell 3’ RNA is able to work with single cells from a variety of origins. Labs have demonstrated success with various cell types from human and mouse (Figure 1) as well as other model organisms (Figure 2).
Figure 1. Labs have demonstrated success using Illumina Single Cell 3’ RNA on various human and mouse cell and tissue types.
Figure 2. Labs have demonstrated success using Illumina Single Cell 3’ RNA on cell types from various model organisms.
Due to the diversity of sample types and use cases, Illumina is unable to provide sample prep protocols for all upstream applications and sample types prior to the single-cell workflow. For finding suitable protocols, one resource that may be utilized is:
This non-Illumina database collects a large number of single cell dissociation techniques.
Users should review the single-cell literature to see what types of isolation methods are commonly used for their sample type for single-cell technologies, and implement all of Illumina’s sample prep recommendations from the Illumina Single Cell user guides and the in addition to their chosen workflow. These recommendations include increasing RNase inhibitors when working with challenging sample types that have high endogenous RNase and/or low RNA content (eg, neutrophils, eosinophils, adipose, pancreas, spleen, macrophages, plants, etc.), as they can be especially sensitive and data outcomes can be improved by including RNase inhibitor. Granulocytes specifically benefit from including RNase inhibitor even in the cell wash steps prior to counting and QC'ing.
For any feedback or questions regarding this article (Illumina Knowledge Article #9642), contact Illumina Technical Support .
