> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/general.md).

# General

- [FAQ](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list.md)
- [Are the Illumina RNA Index Anchors the same in every well of the plate?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005793.md)
- [BLT PF reagent handling for the Illumina DNA PCR Free workflow questions](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000003454.md)
- [BLT PF usage FAQ for Illumina DNA PCR Free](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000003453.md)
- [Can RSB be used as a substitute for RSB + Tween when sequencing on the NextSeq 1000/2000?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005890.md)
- [Can samples be sent to Illumina for sequencing; does Illumina offer sequencing as a service?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005495.md)
- [Consumable FAQ for the Illumina 5 Base Whole Genome Sequencing (WGS) and Enrichment kits](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000009940.md)
- [Consumables and Equipment FAQ for the TruPath Genome assay](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000010166.md)
- [Control (GDMC and SFMC) FAQ for the Illumina 5 Base Whole Genome Sequencing and Enrichment kits](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000009943.md)
- [Design and coverage of the Respiratory Virus Oligos Panel v2 (RVOP v2)](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000003422.md)
- [Does library loading differ between MiSeq Micro and Nano flow cells?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005606.md)
- [FAQs on the Illumina DNA Prep, (M) Tagmentation Library Preparation protocol](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000001260.md)
- [Frequently Asked Questions for Illumina FFPE DNA Prep with Exome 2.5 Enrichment](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000009485.md)
- [General FAQ for Illumina Rapid RNA kits](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000010209.md)
- [General FAQ for the Illumina 5 Base WGS and Enrichment kits](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000009939.md)
- [General workflow FAQ for the Illumina 5 Base WGS and Enrichment kits](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000009946.md)
- [How many samples can be prepared with the Illumina Exome 2.5 panel?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000009598.md)
- [How much PhiX to spike in for well balanced, base diverse libraries?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000006972.md)
- [How to pool Illumina DNA Prep with Exome 2.5 libraries on the NextSeq series and NovaSeq 6000?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000006988.md)
- [How to stagger sequences or heterogeneity spacers introduce diversity for amplicon libraries?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005581.md)
- [Hybridization Buffer (HT1) and Resuspension Buffer (RSB) alternatives and purchasing availability](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005040.md)
- [IDT for Illumina TruSeq Unique Dual (UD) Index kit FAQs for kit configurations and usage](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000001878.md)
- [Information about the TruSeq index adapter plate fixture](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005724.md)
- [Information and protocol for the Illumina 16S Metagenomics Sequencing Library Preparation workflow](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005774.md)
- [Information on the Illumina DNA/RNA UD Indexes](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000008384.md)
- [Input recommendations for Illumina Rapid RNA Kits](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000010212.md)
- [Kits, consumables, and reagent stability FAQ for Illumina Rapid RNA Kits](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000010210.md)
- [Link to the Sequencing Methods Explorer](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000006080.md)
- [Plate layout for the Illumina DNA/RNA UD Indexes (v3)](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000008364.md)
- [Product page and support page links for Illumina DNA Prep with Enrichment (S) Tagmentation](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005054.md)
- [Product page and support page links for Illumina RNA Prep with Enrichment, (L) Tagmentation](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005216.md)
- [QC steps FAQ for Illumina Rapid RNA Kits](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000010214.md)
- [Sequences and plate positions for the Illumina Unique Dual Indexes, LT (48 Indexes, 48 Samples)](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000008386.md)
- [Sequencing and run setup FAQ for Illumina Rapid RNA Kits](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000010215.md)
- [Should indexes be read when sequencing a single library?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000006091.md)
- [What do (S), (M), and (L) mean in Illumina library prep kit names?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000006971.md)
- [What is a Single Read (SR), Paired End (PE) reads, and read requirements for sequencing?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000009547.md)
- [What is the concentration of Illumina adapters and primers?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005459.md)
- [What is the difference between a single and dual indexed library?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005669.md)
- [What is the minimum library size that can be sequenced?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000006540.md)
- [Where are the sequences and plate positions of Illumina indexes?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000007378.md)
- [Which TruSeq index kits come in plate or tube format?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000002332.md)
- [Which computer operating systems are compatible with Illumina Experiment Manager (IEM)?](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000005722.md)
- [Workflow FAQ for Illumina Rapid RNA Kits](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000010213.md)
- [Workflow and Sequencing recommendations FAQ for TruPath Genome](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-faq-list/000010168.md)
- [Reference Material](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list.md)
- [Adapter dimers的形成原因、影响及去除方法](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000007418.md)
- [AmpliSeq for Illumina Dermatology Research Panel v2 product page, support page, and manifest links](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000005192.md)
- [Amplicon Sequencing Introduction Support Webinar Video](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000008059.md)
- [Bead handling best practices](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000002136.md)
- [Best Practices for Ribo Zero Depletion using the TruSeq Stranded Total RNA Protocol](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000001538.md)
- [Best practices for LNB1 handling in bead based normalization for TruSight Oncology 500 workflows](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000002721.md)
- [Best practices for handling magnetic beads used with Illumina RNA library preparation kits](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000003305.md)
- [Best practices for storage of bead normalized Nextera XT libraries](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000001146.md)
- [Best practices for using Sodium Hydroxide with Illumina sequencing systems](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000006560.md)
- [Choosing a Library Loading Concentration for Illumina Sequencing Video](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000006959.md)
- [Custom primer requirements for the Illumina DNA PCR Free Prep, Tagmentation kit](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000003343.md)
- [Fusion Genes kits for detection and lists of targeted genes](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000003067.md)
- [How Do I Optimize Amplicon Sequencing Data? Part 1 Video](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000005726.md)
- [How Do I Optimize Amplicon Sequencing Data? Part 2 Video](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000005742.md)
- [How short inserts affect sequencing performance](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000003874.md)
- [How to Use the Illumina Library Prep Kit Selector Video](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000006960.md)
- [How to find a Safety Data Sheet](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000003798.md)
- [How to use the Illumina Sequencing Coverage Calculator Video](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000005861.md)
- [Illumina DNA Prep library preparation for the MiSeq i100 Series 25M Reagent Kit (1000 cycles)](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000010102.md)
- [Illumina Single Cell 3' RNA Prep, T20 Workflow (Video)](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000010035.md)
- [Illumina library applications for 2x500 bp sequencing on the MiSeq i100](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000009958.md)
- [Index content for the TruSeq DNA and RNA Single Index kits, Sets A and B](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000002510.md)
- [Indexed Sequencing Overview for Paired End Flow Cells](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000002099.md)
- [Information on the Illumina Tagment DNA TDE1 Enzyme and Buffer Kits](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000002753.md)
- [Input recommendations and considerations for Illumina DNA Prep with Enrichment (S) Tagmentation](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000001554.md)
- [Library Prep Bead Handling Workflows Best Practices LNB1/2/3 (Video)](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000010037.md)
- [Library Prep Bead Handling Workflows Best Practices SMB (Video)](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000010038.md)
- [Library Prep Bead Handling Workflows Best Practices SPB/IPB Video](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000010040.md)
- [Library denaturation and compatibility for the iSeq 100 system](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000001705.md)
- [Metagenomics Part I Introduction to Library Preparation and Sequencing Support Webinar Video](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000008057.md)
- [Optimal Cluster Density Best Practices Video](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000005778.md)
- [PhiX Control v3文库是什么，它在Illumina下一代测序中起到什么作用？](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000007130.md)
- [Plate layout for TruSeq Unique Dual (UD) indexes](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000002337.md)
- [Read Depth Requirements for Illumina Rapid RNA kits](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000010216.md)
- [Recommendations for sequencing Illumina 16S libraries with the NextSeq 1000/2000 600 cycle kits](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000007461.md)
- [Recommended quality control of FFPE samples for Illumina FFPE supported library preparation kits](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000001941.md)
- [Resources for Illumina Rapid RNA kits](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000010220.md)
- [Resources for getting started with Illumina Next Generation Sequencing (NGS)](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000002171.md)
- [Respiratory Virus Enrichment Kit sequencing recommendations and public data resources](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000003426.md)
- [Sequences to use for adapter trimming](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000001314.md)
- [Sequencing FFPE Samples Introduction Support Webinar Video](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000008050.md)
- [Sequencing Method Explorer：哪种二代测序（NGS）方法最适合我的研究？](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000007136.md)
- [Spiking custom primers into the Illumina sequencing primers](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000001542.md)
- [Understanding unique dual indexes (UDI) and associated library prep kits](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000002344.md)
- [Using Sequencing Method Explorer to identify suitable NGS methods for research](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000001245.md)
- [Why pooling libraries at the same concentration does not change the final pool concentration](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000008258.md)
- [i7 and i5 indexes in each Nextera XT index kit](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000002230.md)
- [了解唯一双标签（UDI）与相关建库试剂盒](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000007133.md)
- [将定制引物掺入到Illumina测序引物中](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000007451.md)
- [手动文库浓度标准化最佳操作](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000007111.md)
- [是否可以混合不同类型的文库并同时进行测序？](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000007309.md)
- [最小化 TruSeq Stranded Total RNA 文库中rRNA污染的最佳操作](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000007416.md)
- [末端接头剪切（adapter trimming）时需要用到的序列](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-reference_material-list/000007122.md)
- [Troubleshooting](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-troubleshooting-list.md)
- [Library QC and Troubleshooting with the Bioanalyzer and Fragment Analyzer Support Webinar Video](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-troubleshooting-list/000008041.md)
- [Preventing Contamination Expert Video Tip Video](https://knowledge.illumina.com/library-preparation/general/library-preparation-general-troubleshooting-list/000005747.md)


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