# General

- [FAQ](/library-preparation/general/library-preparation-general-faq-list.md)
- [Can Illumina RNA Prep with Enrichment library prep and enrichment reagents be purchased separately?](/library-preparation/general/library-preparation-general-faq-list/000006623.md)
- [Can PhiX and denatured PhiX be stored and used again?](/library-preparation/general/library-preparation-general-faq-list/000005128.md)
- [Can RSB be used as a substitute for RSB + Tween 20 when sequencing on the NextSeq 1000/2000?](/library-preparation/general/library-preparation-general-faq-list/000005890.md)
- [Can library preparation reagents be stored in a frost free freezer?](/library-preparation/general/library-preparation-general-faq-list/000005798.md)
- [Can samples be sent to Illumina for sequencing; does Illumina offer sequencing as a service?](/library-preparation/general/library-preparation-general-faq-list/000005495.md)
- [Can wells in index plates be used more than once?](/library-preparation/general/library-preparation-general-faq-list/000005240.md)
- [Catalog/Reference numbers for IDT for Illumina DNA/RNA UD and Illumina DNA/RNA UD Indexes](/library-preparation/general/library-preparation-general-faq-list/000008026.md)
- [Denature and dilution FAQ for Illumina DNA PCR Free](/library-preparation/general/library-preparation-general-faq-list/000003444.md)
- [Does library loading differ between MiSeq Micro and Nano flow cells?](/library-preparation/general/library-preparation-general-faq-list/000005606.md)
- [How much PhiX to spike in for well balanced, base diverse libraries?](/library-preparation/general/library-preparation-general-faq-list/000006972.md)
- [How to perform library heat denaturation (heat shock) before sequencing?](/library-preparation/general/library-preparation-general-faq-list/000005129.md)
- [How to prepare < 2 nM libraries for sequencing on NovaSeq X/X Plus](/library-preparation/general/library-preparation-general-faq-list/000009000.md)
- [Hybridization Buffer (HT1) and Resuspension Buffer (RSB) alternatives and purchasing availability](/library-preparation/general/library-preparation-general-faq-list/000005040.md)
- [IDT for Illumina TruSeq Unique Dual (UD) Index kit FAQs for kit configurations and usage](/library-preparation/general/library-preparation-general-faq-list/000001878.md)
- [Indexed Sequencing workflows with 5' to 3' directionality](/library-preparation/general/library-preparation-general-faq-list/000005679.md)
- [Information about the TruSeq index adapter plate fixture](/library-preparation/general/library-preparation-general-faq-list/000005724.md)
- [Introducing the Illumina DNA/RNA UD Indexes](/library-preparation/general/library-preparation-general-faq-list/000008384.md)
- [Link to the Sequencing Methods Explorer](/library-preparation/general/library-preparation-general-faq-list/000006080.md)
- [Plate layout for the Illumina DNA/RNA UD Indexes (v3)](/library-preparation/general/library-preparation-general-faq-list/000008364.md)
- [Product page and support page links for Illumina DNA Prep with Enrichment (S) Tagmentation](/library-preparation/general/library-preparation-general-faq-list/000005054.md)
- [Product page and support page links for Illumina RNA Prep with Enrichment, (L) Tagmentation](/library-preparation/general/library-preparation-general-faq-list/000005216.md)
- [Product page and support page links for TruSeq DNA Exome](/library-preparation/general/library-preparation-general-faq-list/000005343.md)
- [Product page and support page links for TruSeq Methyl Capture EPIC](/library-preparation/general/library-preparation-general-faq-list/000005242.md)
- [Product page and support page links for TruSight Cancer bundled with TruSight Rapid Capture](/library-preparation/general/library-preparation-general-faq-list/000005345.md)
- [Product page and support page links for TruSight Myeloid](/library-preparation/general/library-preparation-general-faq-list/000005346.md)
- [Sequences and plate positions for the Illumina Unique Dual Indexes, LT (48 Indexes, 48 Samples)](/library-preparation/general/library-preparation-general-faq-list/000008386.md)
- [Should index reads be performed if only one library is being sequenced?](/library-preparation/general/library-preparation-general-faq-list/000006541.md)
- [Should indexes be read when sequencing a single library?](/library-preparation/general/library-preparation-general-faq-list/000006091.md)
- [What are the Illumina sequencing primer sequences?](/library-preparation/general/library-preparation-general-faq-list/000007129.md)
- [What do (S), (M), and (L) mean in Illumina library prep kit names?](/library-preparation/general/library-preparation-general-faq-list/000006971.md)
- [What is a Single Read (SR), Paired End (PE) reads, and read requirements for sequencing?](/library-preparation/general/library-preparation-general-faq-list/000009547.md)
- [What is the concentration of Illumina adapters and primers?](/library-preparation/general/library-preparation-general-faq-list/000005459.md)
- [What is the difference between a single and dual indexed library?](/library-preparation/general/library-preparation-general-faq-list/000005669.md)
- [What is the minimum library size that can be sequenced?](/library-preparation/general/library-preparation-general-faq-list/000006540.md)
- [Where are the sequences and plate positions of Illumina indexes?](/library-preparation/general/library-preparation-general-faq-list/000007378.md)
- [Where to find the reference sequence used for phiX alignment?](/library-preparation/general/library-preparation-general-faq-list/000005582.md)
- [Which TruSeq index kits come in plate or tube format?](/library-preparation/general/library-preparation-general-faq-list/000002332.md)
- [Which computer operating systems are compatible with Illumina Experiment Manager (IEM)?](/library-preparation/general/library-preparation-general-faq-list/000005722.md)
- [Reference Material](/library-preparation/general/library-preparation-general-reference_material-list.md)
- [Adapter dimers causes, effects, and how to remove them](/library-preparation/general/library-preparation-general-reference_material-list/000001911.md)
- [Adapter dimers的形成原因、影响及去除方法](/library-preparation/general/library-preparation-general-reference_material-list/000007418.md)
- [Amplicon Sequencing Introduction Support Webinar Video](/library-preparation/general/library-preparation-general-reference_material-list/000008059.md)
- [Bead handling best practices](/library-preparation/general/library-preparation-general-reference_material-list/000002136.md)
- [Bead types in Illumina library preparation kits](/library-preparation/general/library-preparation-general-reference_material-list/000003342.md)
- [Best practices for library quantification](/library-preparation/general/library-preparation-general-reference_material-list/000003750.md)
- [Best practices for manually normalizing library concentrations](/library-preparation/general/library-preparation-general-reference_material-list/000001252.md)
- [Best practices for using Sodium Hydroxide with Illumina sequencing systems](/library-preparation/general/library-preparation-general-reference_material-list/000006560.md)
- [Bubble products in sequencing libraries: causes, identification, and workflow recommendations](/library-preparation/general/library-preparation-general-reference_material-list/000001918.md)
- [Choosing a Library Loading Concentration for Illumina Sequencing Video](/library-preparation/general/library-preparation-general-reference_material-list/000006959.md)
- [Compatible and recommended Illumina library types for the NovaSeq 6000 sequencing platform](/library-preparation/general/library-preparation-general-reference_material-list/000001541.md)
- [Considerations when migrating non Illumina libraries between sequencing platforms](/library-preparation/general/library-preparation-general-reference_material-list/000001478.md)
- [DNA/RNA isolation considerations when using Illumina library prep kits](/library-preparation/general/library-preparation-general-reference_material-list/000001249.md)
- [Final library trace examples for the Illumina Library Prep Kits](/library-preparation/general/library-preparation-general-reference_material-list/000006037.md)
- [Fusion Genes kits for detection and lists of targeted genes](/library-preparation/general/library-preparation-general-reference_material-list/000003067.md)
- [How Can I Tell If I Sequenced Through the Insert? Part 1 Video](/library-preparation/general/library-preparation-general-reference_material-list/000005705.md)
- [How Can I Tell If I Sequenced Through the Insert? Part 2 Video](/library-preparation/general/library-preparation-general-reference_material-list/000005725.md)
- [How Do I Achieve Consistent Quantitation? Part 1 Video](/library-preparation/general/library-preparation-general-reference_material-list/000005681.md)
- [How Do I Achieve Consistent Quantitation? Part 2 Video](/library-preparation/general/library-preparation-general-reference_material-list/000005683.md)
- [How Do I Optimize Amplicon Sequencing Data? Part 1 Video](/library-preparation/general/library-preparation-general-reference_material-list/000005726.md)
- [How Do I Optimize Amplicon Sequencing Data? Part 2 Video](/library-preparation/general/library-preparation-general-reference_material-list/000005742.md)
- [How short inserts affect sequencing performance](/library-preparation/general/library-preparation-general-reference_material-list/000003874.md)
- [How to Plan Your First Sequencing Project Support Webinar Video](/library-preparation/general/library-preparation-general-reference_material-list/000008042.md)
- [How to Use the Illumina Library Prep Kit Selector Video](/library-preparation/general/library-preparation-general-reference_material-list/000006960.md)
- [How to find a Safety Data Sheet](/library-preparation/general/library-preparation-general-reference_material-list/000003798.md)
- [How to mitigate index hopping](/library-preparation/general/library-preparation-general-reference_material-list/000001928.md)
- [How to see more content and products relevant to your field of interest](/library-preparation/general/library-preparation-general-reference_material-list/000002704.md)
- [How to use the Illumina Sequencing Coverage Calculator Video](/library-preparation/general/library-preparation-general-reference_material-list/000005861.md)
- [IDT for Illumina DNA/RNA Unique Dual Index Compatibility on the MiniSeq](/library-preparation/general/library-preparation-general-reference_material-list/000002045.md)
- [Illumina library applications for 2x500 bp sequencing on the MiSeq i100](/library-preparation/general/library-preparation-general-reference_material-list/000009958.md)
- [Index content for the TruSeq DNA and TruSeq RNA Single Index kits, Sets A and B](/library-preparation/general/library-preparation-general-reference_material-list/000002510.md)
- [Indexed Sequencing Overview for Paired End Flow Cells](/library-preparation/general/library-preparation-general-reference_material-list/000002099.md)
- [Introduction to Illumina DesignStudio Support Webinar Video](/library-preparation/general/library-preparation-general-reference_material-list/000008056.md)
- [Is it possible to pool different library types in the same sequencing run?](/library-preparation/general/library-preparation-general-reference_material-list/000003284.md)
- [Library Prep Bead Handling Workflows Best Practices LNB1/2/3 (Video)](/library-preparation/general/library-preparation-general-reference_material-list/000010037.md)
- [Library Prep Bead Handling Workflows Best Practices SMB (Video)](/library-preparation/general/library-preparation-general-reference_material-list/000010038.md)
- [Library Prep Bead Handling Workflows Best Practices SPB/IPB Video](/library-preparation/general/library-preparation-general-reference_material-list/000010040.md)
- [Library denaturation and compatibility for the iSeq 100 system](/library-preparation/general/library-preparation-general-reference_material-list/000001705.md)
- [Library quantification and quality control quick reference guide](/library-preparation/general/library-preparation-general-reference_material-list/000001247.md)
- [Metagenomics Part I Introduction to Library Preparation and Sequencing Support Webinar Video](/library-preparation/general/library-preparation-general-reference_material-list/000008057.md)
- [Optimal Cluster Density Best Practices Video](/library-preparation/general/library-preparation-general-reference_material-list/000005778.md)
- [PhiX Control v3文库是什么，它在Illumina下一代测序中起到什么作用？](/library-preparation/general/library-preparation-general-reference_material-list/000007130.md)
- [Plate layout for TruSeq Combinatorial Dual (CD) indexes](/library-preparation/general/library-preparation-general-reference_material-list/000002335.md)
- [Plate layout for TruSeq Unique Dual (UD) indexes](/library-preparation/general/library-preparation-general-reference_material-list/000002337.md)
- [Plate layouts for IDT for Illumina DNA/RNA UD Index kits, Sets A, B, C, and D](/library-preparation/general/library-preparation-general-reference_material-list/000002338.md)
- [Recommended quality control of FFPE samples for Illumina FFPE supported library preparation kits](/library-preparation/general/library-preparation-general-reference_material-list/000001941.md)
- [Resources for getting started with Illumina Next Generation Sequencing (NGS)](/library-preparation/general/library-preparation-general-reference_material-list/000002171.md)
- [Sequences to use for adapter trimming](/library-preparation/general/library-preparation-general-reference_material-list/000001314.md)
- [Sequencing FFPE Samples Introduction Support Webinar Video](/library-preparation/general/library-preparation-general-reference_material-list/000008050.md)
- [Sequencing Method Explorer：哪种二代测序（NGS）方法最适合我的研究？](/library-preparation/general/library-preparation-general-reference_material-list/000007136.md)
- [Sequencing primer compatibility and recommended library kits for the NextSeq 1000/2000 platforms](/library-preparation/general/library-preparation-general-reference_material-list/000005905.md)
- [Sequencing primer compatibility of Illumina libraries and kit types for NextSeq 500/550 and MiniSeq](/library-preparation/general/library-preparation-general-reference_material-list/000001500.md)
- [Sequencing primer compatibility of Illumina libraries and library types for the iSeq 100](/library-preparation/general/library-preparation-general-reference_material-list/000001669.md)
- [Sequencing primer compatibility of Illumina libraries and recommended library kit for the MiSeq](/library-preparation/general/library-preparation-general-reference_material-list/000001494.md)
- [Spiking custom primers into the Illumina sequencing primers](/library-preparation/general/library-preparation-general-reference_material-list/000001542.md)
- [Understanding the differences between Illumina index chemistries and anchor function](/library-preparation/general/library-preparation-general-reference_material-list/000006561.md)
- [Understanding unique dual indexes (UDI) and associated library prep kits](/library-preparation/general/library-preparation-general-reference_material-list/000002344.md)
- [Using Sequencing Method Explorer to identify suitable NGS methods for research](/library-preparation/general/library-preparation-general-reference_material-list/000001245.md)
- [What is the PhiX Control v3 Library and what is its function in Illumina Next Generation Sequencing](/library-preparation/general/library-preparation-general-reference_material-list/000001545.md)
- [Why pooling libraries at the same concentration does not change the final pool concentration](/library-preparation/general/library-preparation-general-reference_material-list/000008258.md)
- [了解唯一双标签（UDI）与相关建库试剂盒](/library-preparation/general/library-preparation-general-reference_material-list/000007133.md)
- [将定制引物掺入到Illumina测序引物中](/library-preparation/general/library-preparation-general-reference_material-list/000007451.md)
- [手动文库浓度标准化最佳操作](/library-preparation/general/library-preparation-general-reference_material-list/000007111.md)
- [是否可以混合不同类型的文库并同时进行测序？](/library-preparation/general/library-preparation-general-reference_material-list/000007309.md)
- [最小化 TruSeq Stranded Total RNA 文库中rRNA污染的最佳操作](/library-preparation/general/library-preparation-general-reference_material-list/000007416.md)
- [末端接头剪切（adapter trimming）时需要用到的序列](/library-preparation/general/library-preparation-general-reference_material-list/000007122.md)
- [Troubleshooting](/library-preparation/general/library-preparation-general-troubleshooting-list.md)
- [Library QC and Troubleshooting with the Bioanalyzer and Fragment Analyzer Support Webinar Video](/library-preparation/general/library-preparation-general-troubleshooting-list/000008041.md)
- [Preventing Contamination Expert Video Tip Video](/library-preparation/general/library-preparation-general-troubleshooting-list/000005747.md)
- [Troubleshooting elevated PhiX alignment in sequencing runs](/library-preparation/general/library-preparation-general-troubleshooting-list/000009236.md)