> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/rna-library-prep.md).

# RNA Library Prep

- [Reference Material](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list.md)
- [Anchor ligation best practices for Illumina Stranded mRNA and Total RNA Prep Ligation workflows](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000003309.md)
- [Best practices to minimize rRNA contamination in TruSeq Stranded Total RNA libraries](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000001921.md)
- [Considerations for RNA Seq read length and coverage](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000001243.md)
- [General best practices for Illumina RNA library preparation kits](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000003306.md)
- [PhiX requirements for sequencing Illumina Stranded RNA libraries](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000003708.md)
- [Product page and support page for Illumina Stranded mRNA Prep, Ligation](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000005220.md)
- [RNA Sequencing Part I Intro to Illumina's RNA Library Preparation Workflows Support Webinar Video](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000008044.md)
- [RNA Sequencing Part II Best Practices for Illumina's RNA Prep Protocols Support Webinar Video](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000008043.md)
- [RNA handling best practices for Illumina RNA library preparation kits](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000003304.md)
- [RNA测序读长及测序深度的选择](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000007414.md)
- [Small RNA Seq Part I Best Practices Support Webinar Video](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000008048.md)
- [Small RNA Seq Part II Best Practices Support Webinar Video](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000008049.md)
- [Species compatibility table for Ribo Zero Human/Mouse/Rat and Ribo Zero Gold](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000002095.md)
- [Species compatibility table for Ribo Zero Plant](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000002096.md)
- [Types of small RNA and the need for a separate small RNA kit](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000002864.md)
- [Which reads map to each strand in Stranded RNA workflows, and how is strandedness achieved?](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000002238.md)
- [rRNA Depletion using Illumina Ribo Zero Plus](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-reference_material-list/000005899.md)
- [FAQ](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list.md)
- [Are technical replicates needed for TruSeq Stranded RNA Seq experiments?](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000002863.md)
- [Directional sequencing and strandedness for Illumina/TruSeq Stranded Total RNA and mRNA libraries](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000001233.md)
- [Frequently asked questions regarding the Ribo Zero Plant and Globin kits.](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000001153.md)
- [General information on Ribo Zero Plus Microbiome](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000006974.md)
- [Illumina Stranded Total RNA Prep, Ligation with Ribo Zero Plus FAQs](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000003152.md)
- [Illumina Stranded mRNA Prep Ligation General FAQs](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000003151.md)
- [Illumina Stranded mRNA Prep Ligation Input FAQs](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000003289.md)
- [Illumina Stranded mRNA Prep Ligation Protocol FAQs](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000003290.md)
- [Index and UMI information for the Illumina miRNA prep kit](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000009893.md)
- [Input FAQs for the Illumina TruSight RNA Pan Cancer library preparation kit](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000003256.md)
- [Input recommendations and considerations for COVIDSeq (RUO) library preparation](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000005433.md)
- [Input recommendations for the Illumina miRNA prep kit](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000009891.md)
- [Library prep workflow frequently asked questions for the Illumina miRNA prep kit](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000009892.md)
- [Library quantification and QC for the Illumina miRNA prep kit](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000009894.md)
- [Product page and support page links for Illumina Stranded Total RNA with Ribo Zero Plus](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000005217.md)
- [Product page and support page links for TruSeq Stranded Total RNA](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000005344.md)
- [Protocol FAQs for Illumina Stranded Total RNA Prep Ligation with Ribo Zero Plus](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000003286.md)
- [Ribo Zero Plus rRNA Depletion Kit FAQs](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000002329.md)
- [Sequencing FAQs for Illumina Stranded RNA libraries](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000003291.md)
- [Sequencing TruSeq Small RNA libraries](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000001399.md)
- [Sequencing frequently asked questions for the Illumina miRNA prep kit](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000009895.md)
- [TruSeq RNA v2 product and support page links](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000005656.md)
- [TruSeq Small RNA Product and Support Pages](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000005657.md)
- [TruSeq Small RNA alternative T4 RNA Ligase 2, Deletion Mutant supplier](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000008542.md)
- [TruSeq Small RNA input best practices](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000001397.md)
- [TruSeq Stranded RNA bead clean up and library size information](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000001155.md)
- [TruSeq Stranded RNA kit storage and freeze/thaw recommendations](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000001152.md)
- [TruSeq Stranded RNA library preparation input requirements](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000001154.md)
- [TruSeq Stranded mRNA Product and Support Pages](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000005653.md)
- [What are the main differences between TruSeq Stranded and Illumina Stranded kits?](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000005928.md)
- [What volume of TruSeq RNA UD indexes should be used during TruSeq Stranded RNA library preparation?](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000005769.md)
- [mRNA purification and rRNA removal information for TruSeq Stranded RNA workflows](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-faq-list/000001232.md)
- [Troubleshooting](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-troubleshooting-list.md)
- [How to assess rRNA depletion in the TruSeq Stranded Total RNA workflow](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-troubleshooting-list/000002918.md)
- [Impact of RNA degradation on mRNA seq data](https://knowledge.illumina.com/library-preparation/rna-library-prep/library-preparation-rna-library-prep-troubleshooting-list/000002731.md)


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