What is the maximum cell size that can be captured in Illumina Single Cell kits?

What is the maximum cell size that can be captured in Illumina Single Cell kits?

The exact upper limit for cell size has not been fully established at this time; however, Illumina Single Cell kits have successfully captured cells up to 60 µm diameter in size.

• For cells ≥ 60 µm, reducing the cell input number may improve capture efficiency and minimize background noise.

• For cells > 80 µm, isolating nuclei and using nuclei as input instead of whole cells is recommended.

See the following resources for nuclei preparation best practices for Illumina Single Cell:

Best practices for using Illumina Single Cell Nuclei Isolation Kits for Illumina Single Cell kits

Best Practices for Nuclei Isolation with alternative nuclei isolation protocols for Illumina Single Cell

These recommendations help to optimize capture efficiency in addition to minimizing background noise and maximizing sequencing data quality.

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