What's New

Articles published to Illumina Knowledge in the past 2 months:

• A quick visualization method to help determine the sex of given samples in a GenomeStudio Methylation project (April 11, 2025)

• Activating TeamViewer on the MiSeq i100 Series Instruments (April 11, 2025)

• Assessing cDNA QC in Illumina Single Cell 3’ RNA Prep (April 11, 2025)

• Assessing library QC in Illumina Single Cell 3’ RNA Prep (April 11, 2025)

• Best Practices for Nuclei Isolation with alternative nuclei isolation protocols for Illumina Single Cell (April 11, 2025)

• Best practices for cell preparation for Illumina Single Cell prep (April 11, 2025)

• Best practices for using Illumina Single Cell Nuclei Isolation Kits for Illumina Single Cell kits (April 11, 2025)

• Can vortexing damage cells/nuclei when generating emulsions in the Illumina Single Cell workflow? (April 11, 2025)

• Cell input and volume loading recommendations for Illumina Single Cell 3’ RNA Prep (April 11, 2025)

• Cloud Analysis Errors using Sample Sheet Workflow on the MiSeq i100 Series (April 11, 2025)

• Considerations for Index color balancing on the MiSeq i100 Series (April 11, 2025)

• Differences between V4.0PLUS PIPseq and Illumina Single Cell (formerly PIPseq V) (April 11, 2025)

• Does TruSight Oncology 500 (TSO500) use a segmentation algorithm to determine the genomic coordinates of a copy number call? (April 11, 2025)

• Does the DRAGEN pipeline support VQSR (Variant Quality Score Recalibration)? (April 11, 2025)

• Frequently Asked Questions for Illumina FFPE DNA Prep with Exome 2.5 Enrichment (April 11, 2025)

• Handling and disposal information for used reagents on the MiSeq i100 Series (April 11, 2025)

• How to Access the Linux Terminal on the MiSeq i100 Series Instruments (April 11, 2025)

• How to Disable Cloud Login Feature on the MiSeq i100 and NovaSeq X Series (April 11, 2025)

• How to Restart File Transfer on the MiSeq i100 Series (April 11, 2025)

• How to Restart File Transfer on the NovaSeq X Series (April 11, 2025)

• How to Setup a Remote Connection to Illumina Run Manager on the MiSeq i100 Series Instruments (April 11, 2025)

• How to change passwords for the Control Software accounts on the MiSeq i100 Series Instrument (April 11, 2025)

• How to perform System Check on the NovaSeq X Series (April 11, 2025)

• How to set up a PhiX validation run on MiSeq i100 Series (April 11, 2025)

• How to use the Log Bundler v1.3 Application on NovaSeq X Series (April 11, 2025)

• Illumina Single Cell 3' RNA Preparation library sequencing recommendations and guidelines (April 11, 2025)

• Illumina Single Cell 3’ RNA Prep cDNA QC best practices (April 11, 2025)

• Illumina Single Cell 3’ RNA Prep library QC best practices (April 11, 2025)

• Impact of RNA degradation on mRNA seq data (April 11, 2025)

• Is it possible to add or remove fields in DRAGEN Array Cloud Genotyping Final Report? (April 11, 2025)

• Mitigating Instrument Platform Services Errors on the MiSeq i100 Series (April 11, 2025)

• Planning a Manual Mode Run on MiSeq i100 Series (April 11, 2025)

• RTA4 Q Score Bin Ranges on the MiSeq i100 Series (April 11, 2025)

• Recommended Network Security Allow List for the MiSeq i100 Series (April 11, 2025)

• Representative cDNA QC traces for Illumina Single Cell 3’ RNA Prep (April 11, 2025)

• Representative library QC traces for Illumina Single Cell 3’ RNA Prep (April 11, 2025)

• Run Mode options for the MiSeq i100 Series (April 11, 2025)

• Sample Enrichment Best Practices for Illumina Single Cell 3' RNA Prep (April 11, 2025)

• Sample Sheet Import Errors on the NextSeq 1000/2000 (April 11, 2025)

• Selecting cycle numbers for High and Low RNA sample types with Illumina Single Cell RNA prep kits (April 11, 2025)

• Shelf life of Illumina Single Cell 3' RNA Prep kits (April 11, 2025)

• Single sample group formation to reduce GenomeStudio Methylation Module crashing during analysis (April 11, 2025)

• Specifications for the MiSeq i100 Series Instruments (April 11, 2025)

• Spiking custom primers into the Illumina sequencing primers for MiSeq i100 Series (April 11, 2025)

• The importance of pilot studies for Illumina Single Cell RNA Preparation prior to workflow changes (April 11, 2025)

• Troubleshooting APIException: Bad Request Errors when setting Default Output Folder on the MiSeq i100 Series (April 11, 2025)

• Troubleshooting CIM Homing Errors during Initialization on the MiSeq i100 Series (April 11, 2025)

• Troubleshooting Flow Cell Thermal Errors during Pre Run Checks on the MiSeq i100 Series (April 11, 2025)

• Troubleshooting Index Dropouts or No Reads Identified for Single Sample on the MiSeq i100 Series (April 11, 2025)

• Troubleshooting Issues with Waste Level Sensor System Check on the MiSeq i100 Series (April 11, 2025)

• Troubleshooting Local Run Manager originally caused by an internal server error on NextSeq 500/550 (April 11, 2025)

• Troubleshooting Pressure Charging Failures on the MiSeq i100 Series (April 11, 2025)

• Troubleshooting Runs not Uploading to Cloud on the NovaSeq X and MiSeq i100 Series (April 11, 2025)

• Troubleshooting Stalled Cloud Login Screen on the MiSeq i100 Series (April 11, 2025)

• Troubleshooting Touch Screen Not Responding on the MiSeq i100 Series (April 11, 2025)

• Troubleshooting Unable to connect to external storage Errors on the MiSeq i100 Series (April 11, 2025)

• Troubleshooting multiple entries with identical indices error during run setup on NovaSeq X Series (April 11, 2025)

• Using expired reagents on the NovaSeq X Series (April 11, 2025)

• Viral Surveillance Panel (VSP) probes and panel design frequently asked questions (FAQs) (April 11, 2025)

• What are the different BaseSpace Account Types? (April 11, 2025)

• What is a Single Read (SR), Paired End (PE) reads, and read requirements for sequencing? (April 11, 2025)

• Where to find the MAC Address for the MiSeq i100 Series (April 11, 2025)

• Which computer operating systems are compatible with Illumina Experiment Manager (IEM)? (April 11, 2025)

• Why there may be multiple assays targeting the same SNP marker in array manifests (April 11, 2025)

• Troubleshooting Cloud Connectivity Pre Run Check Errors on the MiSeq i100 Series (February 21, 2025)