What's New

Articles published to Illumina Knowledge in the past 2 months:

• Troubleshooting Stalled or Frozen Sequencing Runs on the MiSeq i100 Series (May 21, 2025)

• How many samples can be prepared with the Illumina Exome 2.5 panel? (May 19, 2025)

• Troubleshooting "the owner of workgroup does not have access to the workgroup" message in BaseSpace (May 16, 2025)

• Guidance regarding light seen from the DRAGEN v4 power supply when an unstable power cord is used (May 12, 2025)

• Creating a custom genome for NovaSeq X Series (April 24, 2025)

• How to enable Wi Fi on the iSeq 100 (April 24, 2025)

• Offline DRAGEN License Installation on the MiSeq i100 Series (April 24, 2025)

• Why is Illumina Single Cell sequencing read depth based on input cells? (April 24, 2025)

• 0.8X bead cleanup to remove adapter dimers from Illumina Single Cell 3’ RNA Prep libraries (April 22, 2025)

• Can vortexing damage cells/nuclei when generating emulsions in the Illumina Single Cell workflow? (April 22, 2025)

• Extending cDNA storage time in Illumina Single Cell 3' RNA Prep (April 22, 2025)

• Illumina Single Cell 3’ RNA Prep cDNA QC best practices (April 22, 2025)

• Illumina Single Cell 3’ RNA Prep library QC best practices (April 22, 2025)

• Representative library QC traces for Illumina Single Cell 3’ RNA Prep (April 22, 2025)

• Selecting cycle numbers for High and Low RNA sample types with Illumina Single Cell RNA prep kits (April 22, 2025)

• Shelf life of Illumina Single Cell 3' RNA Prep kits (April 22, 2025)

• Validated USB Storage Devices for NovaSeq X Series Control Software v1.3.1 (April 15, 2025)

• Troubleshooting Regions of Reduced %Passing Filter on NovaSeq X Series (April 11, 2025)

• Cloud Analysis Errors using Sample Sheet Workflow on the MiSeq i100 Series (April 10, 2025)

• Spiking custom primers into the Illumina sequencing primers for MiSeq i100 Series (April 10, 2025)

• Troubleshooting CIM Homing Errors during Initialization on the MiSeq i100 Series (April 10, 2025)

• Mitigating Instrument Platform Services Errors on the MiSeq i100 Series (April 7, 2025)

• What is a Single Read (SR), Paired End (PE) reads, and read requirements for sequencing? (March 31, 2025)

• Assessing cDNA QC in Illumina Single Cell 3’ RNA Prep (March 27, 2025)

• Assessing library QC in Illumina Single Cell 3’ RNA Prep (March 27, 2025)

• Cell input and volume loading recommendations for Illumina Single Cell 3’ RNA Prep (March 27, 2025)

• Does TruSight Oncology 500 (TSO500) use a segmentation algorithm to determine the genomic coordinates of a copy number call? (March 27, 2025)

• Is it possible to add or remove fields in DRAGEN Array Cloud Genotyping Final Report? (March 27, 2025)

• Representative cDNA QC traces for Illumina Single Cell 3’ RNA Prep (March 27, 2025)

• Single sample group formation to reduce GenomeStudio Methylation Module crashing during analysis (March 27, 2025)

• RTA4 Q Score Bin Ranges on the MiSeq i100 Series (March 25, 2025)