What's New

Articles published to Illumina Knowledge in the past 2 months:

• A quick visualization method to help determine the sex of given samples in a GenomeStudio Methylation project (March 24, 2025)

• Activating TeamViewer on the MiSeq i100 Series Instruments (March 24, 2025)

• AmpliSeq Custom DNA Panel product and support pages (March 24, 2025)

• AmpliSeq Custom RNA Panel product and support pages (March 24, 2025)

• AmpliSeq On Demand Panel Product and Support Pages (March 24, 2025)

• Array Manifest Why are there multiple assays targeting the same SNP marker (March 24, 2025)

• Best Practices for Nuclei Isolation with alternative nuclei isolation protocols for Illumina Single Cell (March 24, 2025)

• Best practices for cell preparation for Illumina Single Cell prep (March 24, 2025)

• Best practices for using Illumina Single Cell Nuclei Isolation Kits for Illumina Single Cell kits (March 24, 2025)

• COVIDSeq timing and logistics (March 24, 2025)

• Can Genotype Call (GTC) files be used as input for GenomeStudio? (March 24, 2025)

• Can additional users be added to the Linux interface on the NextSeq 1000/2000? (March 24, 2025)

• Can library preparation reagents be stored in a frost free freezer? (March 24, 2025)

• Can vortexing damage cells/nuclei when generating emulsions in the Illumina Single Cell workflow? (March 24, 2025)

• Considerations for Index color balancing on the MiSeq i100 Series (March 24, 2025)

• Differences between Illumina DNA Prep (M) Tagmentation (Nextera DNA Flex), Nextera DNA, and Nextera XT kits (March 24, 2025)

• Differences between V4.0PLUS PIPseq and Illumina Single Cell (formerly PIPseq V) (March 24, 2025)

• Differences with TruSeq Stranded RNA LS and HS protocols and single and dual index adapters (March 24, 2025)

• Does DRAGEN Array have a Call Rate limit to estimate gender? (March 24, 2025)

• Effects of library quality and indexes on demultiplexing NovaSeq X Series data (March 24, 2025)

• End of Life Schedule for NextSeq 1000 / 2000 SBS Standard Consumables (March 24, 2025)

• Estimating Disk Space Utilization on the MiSeq i100 Series (March 24, 2025)

• Handling and disposal information for used reagents on the MiSeq i100 Series (March 24, 2025)

• How does the Illumina/Nextera tagmentation enzyme make sure that both p5 and p7 adapters are added? (March 24, 2025)

• How to Access the Linux Terminal on the MiSeq i100 Series Instruments (March 24, 2025)

• How to Configure Network Settings for the MiSeq i100 after First Time Setup (March 24, 2025)

• How to Disable Cloud Login Feature on the MiSeq i100 and NovaSeq X Series (March 24, 2025)

• How to Power Cycle the MiSeq i100 Series (March 24, 2025)

• How to Restart File Transfer on the MiSeq i100 Series (March 24, 2025)

• How to Restart File Transfer on the NovaSeq X Series (March 24, 2025)

• How to Setup a Remote Connection to Illumina Run Manager on the MiSeq i100 Series Instruments (March 24, 2025)

• How to Setup the System Health Checks on the MiSeq i100 Series Instruments (March 24, 2025)

• How to Spike in PhiX for the MiSeq i100 Series (March 24, 2025)

• How to Troubleshoot Clustering Failures on the MiSeq i100 Series (March 24, 2025)

• How to Troubleshoot a "no base calls are available for its lane(s)" error in Local Run Manager (March 24, 2025)

• How to find a Safety Data Sheet (March 24, 2025)

• How to perform System Check on the NovaSeq X Series (March 24, 2025)

• How to retrieve the DRAGEN (FPGA) Serial Number for MiSeq i100 Series Systems (March 24, 2025)

• How to upload files to Illumina Kiteworks using command line tools (March 24, 2025)

• How to use the Log Bundler v1.3 Application on NovaSeq X Series (March 24, 2025)

• Illumina Single Cell 3' RNA Preparation library sequencing recommendations and guidelines (March 24, 2025)

• Impact of RNA degradation on mRNA seq data (March 24, 2025)

• Indexed Sequencing workflows with 5' to 3' directionality (March 24, 2025)

• Information about the TruSeq index adapter plate fixture (March 24, 2025)

• Information and protocol for the Illumina 16S Metagenomics Sequencing Library Preparation workflow (March 24, 2025)

• Information and protocol for the Illumina Fungal Metagenomics Sequencing workflow (March 24, 2025)

• Instrument reference images for the MiSeq i100 Series (March 24, 2025)

• Instrument, flow cell, and reagent requirement FAQ for Illumina DNA PCR Free libraries (March 24, 2025)

• Introducing the Illumina NovaSeq X System (March 24, 2025)

• Library storage FAQ for Illumina DNA PCR Free libraries (March 24, 2025)

• Link to the Sequencing Methods Explorer (March 24, 2025)

• Loading Concentration Optimization Recommendations for the MiSeq i100 Series (March 24, 2025)

• Overview of the Fungal Metagenomic Sequencing Demonstrated Protocol (March 24, 2025)

• Qualification/QC FAQ for Illumina DNA PCR Free libraries (March 24, 2025)

• Reagent Stability Guidelines for the MiSeq i100 Series Consumables (March 24, 2025)

• Recommended Network Security Allow List for the MiSeq i100 Series (March 24, 2025)

• Resources for getting started with Illumina Next Generation Sequencing (NGS) (March 24, 2025)

• Run Mode options for the MiSeq i100 Series (March 24, 2025)

• Sample Enrichment Best Practices for Illumina Single Cell 3' RNA Prep (March 24, 2025)

• Sample Sheet Import Errors on the NextSeq 1000/2000 (March 24, 2025)

• Sequencing Illumina Stranded Total RNA and mRNA Libraries on the MiSeq i100 Series (March 24, 2025)

• Sequencing TruSeq Small RNA libraries (March 24, 2025)

• Sequencing depth and read length considerations for TruSeq Stranded RNA libraries (March 24, 2025)

• Size selection and clean up considerations for TruSeq DNA PCR Free library preparation (March 24, 2025)

• The importance of pilot studies for Illumina Single Cell RNA Preparation prior to workflow changes (March 24, 2025)

• Troubleshooting "Additional instrument configuration is required" Issues on the MiSeq i100 Series (March 24, 2025)

• Troubleshooting 'An unexpected error occurred during sequencing' Errors on the MiSeq i100 Series (March 24, 2025)

• Troubleshooting 'EdgeOS You are offline' errors on the MiSeq i100 Series (March 24, 2025)

• Troubleshooting 'Failed to start First Time Setup' Errors on the MiSeq i100 Series Instruments (March 24, 2025)

• Troubleshooting 'One or more unknown settings found' Errors during Run Planning on the NovaSeq X Series (March 24, 2025)

• Troubleshooting BIM SOUR 2 Handle Position Errors During NovaSeq 6000 System Checks (March 24, 2025)

• Troubleshooting Flow Cell Thermal Errors during Pre Run Checks on the MiSeq i100 Series (March 24, 2025)

• Troubleshooting Inaccurate Estimated Completion Time on the MiSeq i100 Series (March 24, 2025)

• Troubleshooting Index Dropouts or No Reads Identified for Single Sample on the MiSeq i100 Series (March 24, 2025)

• Troubleshooting Insufficient Space on External Storage on the MiSeq i100 Series Instruments. (March 24, 2025)

• Troubleshooting Internal Server Error in Cloud Settings on the MiSeq i100 Series (March 24, 2025)

• Troubleshooting Issues with Allocating Disk Space during Pre Run Checks on the NovaSeq X Series (March 24, 2025)

• Troubleshooting Leak Detection Errors on the MiSeq i100 Series (March 24, 2025)

• Troubleshooting Local Run Manager originally caused by an internal server error on NextSeq 500/550 (March 24, 2025)

• Troubleshooting Memory Metal Alloy (MMA) Motion Stalls on the MiSeq i100 Series (March 24, 2025)

• Troubleshooting Pressure Charging Failures on the MiSeq i100 Series (March 24, 2025)

• Troubleshooting Runs not Uploading to Cloud on the NovaSeq X and MiSeq i100 Series (March 24, 2025)

• Troubleshooting Stalled Cloud Login Screen on the MiSeq i100 Series (March 24, 2025)

• Troubleshooting Touch Screen Not Responding on the MiSeq i100 Series (March 24, 2025)

• Troubleshooting Unable to connect to external storage Errors on the MiSeq i100 Series (March 24, 2025)

• Troubleshooting elevated PhiX alignment in sequencing runs (March 24, 2025)

• Troubleshooting multiple entries with identical indices error during run setup on NovaSeq X Series (March 24, 2025)

• TruSeq RNA v2 product and support page links (March 24, 2025)

• TruSeq Small RNA input best practices (March 24, 2025)

• TruSeq Stranded RNA kit storage and freeze/thaw recommendations (March 24, 2025)

• TruSeq Stranded RNA library preparation input requirements (March 24, 2025)

• Unlocking an account or resetting the password on Local Run Manager version 4 (March 24, 2025)

• Updated guidance for using new blue Infinium Staining Set (March 24, 2025)

• Viral Surveillance Panel (VSP) reagents and workflow frequently asked questions (FAQs) (March 24, 2025)

• Wash Flow Cell Use Counter on NovaSeq X Series (March 24, 2025)

• What is the Illumina Lysis Reagent Kit, and when should it be used? (March 24, 2025)

• What is the difference between a single and dual indexed library? (March 24, 2025)

• When will Live %Demux information be available for a MiSeq i100 Series run? (March 24, 2025)

• Where to find the reference sequence used for phiX alignment? (March 24, 2025)

• Which Nextera Index Kits come in plate or tube format? (March 24, 2025)

• Which TruSeq index kits come in plate or tube format? (March 24, 2025)

• Which computer operating systems are compatible with Illumina Experiment Manager (IEM)? (March 24, 2025)

• Why is NextSeq 1000/2000 control software update not available from the Software Update menu? (March 24, 2025)

• mRNA purification and rRNA removal information for TruSeq Stranded RNA workflows (March 24, 2025)

• Troubleshooting Cloud Connectivity Pre Run Check Errors on the MiSeq i100 Series (February 21, 2025)