What's New

Articles published to Illumina Knowledge in the past 2 months:

• Air Filter replacement on the MiSeq i100 (November 12, 2025)

• Consumable FAQ for the Illumina 5 Base Whole Genome Sequencing (WGS) and Enrichment kits (November 11, 2025)

• Control (GDMC and SFMC) FAQ for the Illumina 5 Base Whole Genome Sequencing and Enrichment kits (November 11, 2025)

• Enrichment Panels and DesignStudio FAQ for the Illumina 5 Base WGS and Enrichment kits (November 11, 2025)

• Enrichment workflow FAQ for the Illumina 5 Base DNA Prep with Enrichment kit (November 11, 2025)

• General workflow FAQ for the Illumina 5 Base WGS and Enrichment kits (November 11, 2025)

• Input recommendations for the Illumina 5 Base WGS and Enrichment kits (November 11, 2025)

• Sequencing FAQ for the Illumina 5 Base WGS and Enrichment kits (November 11, 2025)

• UMI and index FAQ for the Illumina 5 Base Whole Genome Sequencing (WGS) and Enrichment kits (November 11, 2025)

• Analysis launch FAQ for the Illumina 5 Base WGS and Enrichment kits (November 5, 2025)

• Analysis pipeline and output FAQ for the Illumina 5 Base WGS and Enrichment kits (November 5, 2025)

• How to invite users to an Enterprise domain workgroup, via a collaborative Enterprise domain account (November 5, 2025)

• Is there a limit to the number of files that can be stored within an ICA project? (November 5, 2025)

• Variant and Methylation calling FAQ for the Illumina 5 Base WGS and Enrichment kits (November 5, 2025)

• Can a 10 Gbps connection support data analysis between the DRAGEN server and storage server? (November 3, 2025)

• Can the DRAGEN system handle concurrent users? (November 3, 2025)

• How to enable DRAGEN to process multiple FASTQ files for the same subject? (November 3, 2025)

• How to list BaseSpace entities using BaseSpace CLI (November 3, 2025)

• Troubleshooting "Permission denied" error when attempting to run BaseSpace CLI on Linux and Mac OS (November 3, 2025)

• Troubleshooting aborted ICA v2 analyses on BaseSpace (November 3, 2025)

• General FAQ for the Illumina 5 Base WGS and Enrichment kits (October 31, 2025)

• Methylation Control Panel FAQ for the Illumina 5 Base WGS and Enrichment kits (October 31, 2025)

• Troubleshooting "Insufficient Resource Permissions" when uploading runs with BaseSpace CLI (October 31, 2025)

• Updating the DRAGEN Microbial Enrichment (DME) Databases on the MiSeq i100 Series (October 31, 2025)

• Windows 10 Upgrade for Illumina Instruments FAQ (October 31, 2025)

• Illumina library applications for 2x500 bp sequencing on the MiSeq i100 (October 27, 2025)

• BCL Convert software output files (October 24, 2025)

• Recommended PhiX Loading for a NextSeq 550 Validation Run (October 23, 2025)

• How to prepare instruments for Natural Disasters (October 22, 2025)

• Troubleshooting Dry cartridge ID mismatch Warning on the MiSeq i100 Series (October 22, 2025)

• How to Store and Prepare Consumables for a NextSeq 1000/2000 XLEAP SBS Run Video (October 21, 2025)

• Troubleshooting "Upload mode has been set to None" Errors on the MiSeq i100 Series (October 21, 2025)

• Troubleshooting Instrument Network Configuration (INC) Service Issues on the MiSeq i100 Series (October 21, 2025)

• How to build a Custom Reference Hashtable for BaseSpace DRAGEN Single Cell RNA Application (October 17, 2025)

• Analysis frequently asked questions for the Illumina miRNA prep kit (October 16, 2025)

• Setting up a sample sheet to send all reads to Undetermined in MiSeq Reporter (October 16, 2025)

• Index and UMI information for the Illumina miRNA prep kit (October 14, 2025)

• Input recommendations for the Illumina miRNA prep kit (October 14, 2025)

• Library prep workflow frequently asked questions for the Illumina miRNA prep kit (October 14, 2025)

• Library quantification and QC for the Illumina miRNA prep kit (October 14, 2025)

• Sequencing frequently asked questions for the Illumina miRNA prep kit (October 14, 2025)

• Instructions to designate an output location when setting up a run in MiSeq Control Software v2.x (October 9, 2025)

• How is the Reproducibility Frequency calculated in GenomeStudio? (October 7, 2025)

• PhiX Indexed Control (1000 Cycle) Product Information (October 7, 2025)

• How the iScan determines beadchip type and which scan settings file to apply (September 29, 2025)

• Ordering the HD/LCG/HTS Multi sample Alignment Fixture vs XT/EX Flow through Chamber Assembly Tray (September 29, 2025)

• Running the Reproducibility and Heritability Report with/without Calculating Errors in GenomeStudio (September 29, 2025)

• Compatible callers for non human references in the DRAGEN Germline App (September 26, 2025)

• How many non G bases are required for a cluster to pass filter on the NextSeq 1000/2000? (September 26, 2025)

• How to Check When a Windows Password Was Last Updated or When It Will Expire (September 26, 2025)

• How to Enable Illumina Proactive on the NextSeq 1000/2000 (September 26, 2025)

• How to Power Down the NovaSeq X Series Instruments for a Planned Power Outage (September 26, 2025)

• How to connect to the Java SOL on DRAGEN v3 Hardware (September 26, 2025)

• How to set up a PhiX validation run on the NextSeq 1000/2000 sequencing platform (September 26, 2025)

• How to update the TLS Certificates on the NovaSeq X Plus instruments (September 26, 2025)

• Resetting the TeFlow Water Re Circulator Air Filter Alarm (September 26, 2025)

• Shipping of the reagent cartridge for the NextSeq 1000/2000 (September 26, 2025)

• Troubleshooting DRAGEN single cell RNA App UMI error in BaseSpace (September 26, 2025)

• Troubleshooting pre run check failure due to "Imaging test failed" on the NextSeq 1000/2000 (September 26, 2025)

• Troubleshooting the large waste bottle is full error on the NovaSeq X Series (September 26, 2025)