What's New

Articles published to Illumina Knowledge in the past 2 months:

• Activating TeamViewer on the MiSeq i100 Series Instruments (January 20, 2025)

• AmpliSeq Custom DNA Panel product and support pages (January 20, 2025)

• AmpliSeq Custom RNA Panel product and support pages (January 20, 2025)

• Are technical replicates needed for TruSeq Stranded RNA Seq experiments? (January 20, 2025)

• Best Practices for Ribo Zero Depletion using the TruSeq Stranded Total RNA Protocol (January 20, 2025)

• Can GenomeStudio 2.0 genotyping projects be merged? (January 20, 2025)

• Can Genotype Call (GTC) files be used as input for GenomeStudio? (January 20, 2025)

• Can I add additional user to the Linux interface on the NextSeq 1000/2000 (January 20, 2025)

• Cleaning NextSeq 1000/2000 cartridge tray in case of reagent leak inside the instrument (January 20, 2025)

• Confirming identities of replicate & matched samples with SNP targeted assays in methylation arrays (January 20, 2025)

• Differences between Illumina DNA Prep (M) Tagmentation (Nextera DNA Flex), Nextera DNA, and Nextera XT kits (January 20, 2025)

• Does DRAGEN Array have a Call Rate limit to estimate gender? (January 20, 2025)

• Effects of library quality and indexes on demultiplexing NovaSeq X Series data (January 20, 2025)

• Emedgene troubleshooting information request form (January 20, 2025)

• End of Life Schedule for NextSeq 1000 / 2000 SBS Standard Consumables (January 20, 2025)

• Estimating Disk Space Utilization on the MiSeq i100 Series (January 20, 2025)

• Frequently asked questions regarding the Ribo Zero Plant and Globin kits (January 20, 2025)

• Handling and Disposal Recommendations for Used Reagents on the MiSeq i100 Series (January 20, 2025)

• How Long Does the Flow Rate Check Take During the MiSeq Pre Run Checks? (January 20, 2025)

• How long can bisulfite converted DNA be stored in 20°C for use in Infinium Methylation arrays? (January 20, 2025)

• How to Access the Linux Terminal on the MiSeq i100 Series Instruments (January 20, 2025)

• How to Configure Network Settings for the MiSeq i100 after First Time Setup (January 20, 2025)

• How to Find the Control Software Version on the NextSeq 500/550 (January 20, 2025)

• How to Power Cycle the MiSeq i100 Series (January 20, 2025)

• How to Restart File Transfer on the MiSeq i100 Series (January 20, 2025)

• How to Restart File Transfer on the NovaSeq X Series (January 20, 2025)

• How to Setup a Remote Connection to Illumina Run Manager on the MiSeq i100 Series Instruments (January 20, 2025)

• How to Setup the System Health Checks on the MiSeq i100 Series Instruments (January 20, 2025)

• How to Troubleshoot Clustering Failures on the MiSeq i100 Series (January 20, 2025)

• How to accept BaseSpace transfer ownership requests in a workgroup context (January 20, 2025)

• How to avoid Ns in small RNA or short reads when running BCL Convert (January 20, 2025)

• How to calculate and import LogRDev into a GenomeStudio Project (January 20, 2025)

• How to calculate the DRAGEN license quota (January 20, 2025)

• How to change passwords for the Control Software accounts on the MiSeq i100 Series Instrument (January 20, 2025)

• How to find a Safety Data Sheet (January 20, 2025)

• How to find the FPGA serial number on a DRAGEN server? (January 20, 2025)

• How to identify when an air filter was changed on the iSeq 100. (January 20, 2025)

• How to manage status.illumina.com subscriptions (January 20, 2025)

• How to obtain Unmethylated and Methylated signal in GenomeStudio for each CpG in MethylationEPIC Array (January 20, 2025)

• How to perform Linux OS updates on the NextSeq 1000/2000 (January 20, 2025)

• How to perform System Check on the NovaSeq X Series (January 20, 2025)

• How to requeue analysis using only an index 2 (i5) read (January 20, 2025)

• How to retrieve the DRAGEN (FPGA) Serial Number for MiSeq i100 Series Systems (January 20, 2025)

• How to select GenTrain version in GenomeStudio 2.0 (January 20, 2025)

• How to set OverrideCycles values for variable index sequence lengths on the NovaSeq X/X Plus (January 20, 2025)

• How to set up a PhiX validation run on MiSeq i100 Series (January 20, 2025)

• How to share BaseSpace Microarray project data (January 20, 2025)

• How to troubleshoot NovaSeq 6000 “A component failed during sequencing” error during a Cloud Outage (January 20, 2025)

• How to troubleshoot a Database Backup Secret has not been set warning in Local Run Manager (January 20, 2025)

• How to update the DRAGEN BIOS version to reduce v4 server fan noise (January 20, 2025)

• How to upload files to Illumina Kiteworks using command line tools (January 20, 2025)

• How to use the Log Bundler Lite application on NovaSeq X Series (January 20, 2025)

• Impact of RNA degradation on mRNA seq data (January 20, 2025)

• Infinium Amplification System (IAS Hamilton) power cycle instructions (January 20, 2025)

• Infinium MethylationEPIC v2.0 Manifest Column Headings (January 20, 2025)

• Information about the TruSeq index adapter plate fixture (January 20, 2025)

• Information and protocol for the Illumina 16S Metagenomics Sequencing Library Preparation workflow (January 20, 2025)

• Information and protocol for the Illumina Fungal Metagenomics Sequencing workflow (January 20, 2025)

• Information on BCL Convert software releases (January 20, 2025)

• Introducing the Illumina NovaSeq X System (January 20, 2025)

• Is a sample sheet required to run DRAGEN Array? (January 20, 2025)

• Link to the Sequencing Methods Explorer (January 20, 2025)

• Loading Concentration Optimization Recommendations for the MiSeq i100 Series (January 20, 2025)

• NextSeq 1000/2000 Control Software upgrade from 1.7.0 to 1.7.1 system integration error (January 20, 2025)

• PhiX requirements for sequencing Illumina Stranded RNA libraries (January 20, 2025)

• Post amplification clean up best practices for Illumina RNA Prep with Enrichment (L) Tagmentation (January 20, 2025)

• Product page and support page links for Illumina Cell Free DNA Prep with Enrichment (January 20, 2025)

• Product page and support page links for Illumina Complete Long Read Prep (ICLR), Human (January 20, 2025)

• Product page and support page links for Illumina Complete Long Reads with Enrichment (ICLRE), Human (January 20, 2025)

• Reagent Stability Guidelines for the MiSeq i100 Series Consumables (January 20, 2025)

• Recommended Network Security Allow List for the MiSeq i100 Series (January 20, 2025)

• Resources for getting started with Illumina Next Generation Sequencing (NGS) (January 20, 2025)

• Run Mode options for the MiSeq i100 Series (January 20, 2025)

• Sample Sheet Import Errors on the NextSeq 1000/2000 (January 20, 2025)

• Secure communication protocols on Illumina systems (January 20, 2025)

• Sequencing Illumina Stranded Total RNA and mRNA Libraries on the MiSeq i100 Series (January 20, 2025)

• Sequencing TruSeq Small RNA libraries (January 20, 2025)

• Serial Number locations on Illumina sequencers (January 20, 2025)

• Shelf life for the COVIDSeq library preparation kit (January 20, 2025)

• Troubleshooting "Additional instrument configuration is required" Issues on the MiSeq i100 Series (January 20, 2025)

• Troubleshooting "Component Failed" errors immediately after Pre Run Checks on the NovaSeq 6000 (January 20, 2025)

• Troubleshooting "Could not find a part of the path" errors on the NextSeq500/550 and MiniSeq (January 20, 2025)

• Troubleshooting "Failed to communicate with RTA after 4 attempts" on the MiniSeq or NextSeq 500/550 (January 20, 2025)

• Troubleshooting "GenerateFASTQ 500 Internal Server Error" in Local Run Manager hosted on the iSeq 100 (January 20, 2025)

• Troubleshooting 'A configuration file cannot be assessed' Warnings during Pre Run Checks on the NovaSeq X Series (January 20, 2025)

• Troubleshooting 'An unexpected error occurred during sequencing' Errors on the MiSeq i100 Series (January 20, 2025)

• Troubleshooting 'Failed to start First Time Setup' Errors on the MiSeq i100 Series Instruments (January 20, 2025)

• Troubleshooting 'Status code 523' Errors in Cloud Settings on the MiSeq i100 Series (January 20, 2025)

• Troubleshooting APIException: Bad Request Errors when setting Default Output Folder on the MiSeq i100 Series (January 20, 2025)

• Troubleshooting BIM SOUR 2 Handle Position Errors During NovaSeq 6000 System Checks (January 20, 2025)

• Troubleshooting Blue Screen errors related to the CrowdStrike update (January 20, 2025)

• Troubleshooting EdgeOS You are offline errors on the MiSeq i100 Series (January 20, 2025)

• Troubleshooting HttpClient.Timeout Errors during Pre Run Checks on the MiSeq i100 Series (January 20, 2025)

• Troubleshooting Inaccurate Estimated Completion Time on the MiSeq i100 Series (January 20, 2025)

• Troubleshooting Insufficient Space on External Storage on the MiSeq i100 Series Instruments. (January 20, 2025)

• Troubleshooting Internal Server Error in Cloud Settings on the MiSeq i100 Series (January 20, 2025)

• Troubleshooting Invalid Role Errors after Post Run Washes on the MiSeq (January 20, 2025)

• Troubleshooting Issues during Instrument Shutdown on the NovaSeq X Series (January 20, 2025)

• Troubleshooting Leak Detection Errors on the MiSeq i100 Series (January 20, 2025)

• Troubleshooting Local Run Manager originally caused by an internal server error on NextSeq 500/550 (January 20, 2025)

• Troubleshooting Memory Metal Alloy (MMA) Motion Stalls on the MiSeq i100 Series (January 20, 2025)

• Troubleshooting Pressure Charging Failures on the MiSeq i100 Series (January 20, 2025)

• Troubleshooting Reagent Compartment Doors Failing to Unlock on the NovaSeq X Series (January 20, 2025)

• Troubleshooting System Configuration errors during Pre Run Checks on the MiSeq i100 Series (January 20, 2025)

• Troubleshooting System File errors during pre run checks on the NextSeq 1000/2000 (January 20, 2025)

• Troubleshooting Unable to connect to external storage Errors on the MiSeq i100 Series (January 20, 2025)

• Troubleshooting elevated PhiX alignment in sequencing runs (January 20, 2025)

• Troubleshooting multiple entries with identical indices error during run setup on NovaSeq X Series (January 20, 2025)

• Troubleshooting the Infinium HD FFPE QC Assay Protocol (January 20, 2025)

• TruSeq RNA v2 product and support page links (January 20, 2025)

• TruSeq Small RNA FAQs: Best practice and FAQ about input (January 20, 2025)

• TruSeq Stranded RNA Kit FAQs: AMPure clean up and library size (January 20, 2025)

• TruSeq Stranded RNA Kit FAQs: Sequencing settings (January 20, 2025)

• TruSeq Stranded RNA Kit FAQs: differences from TruSeq RNA Library Prep Kit v2 (January 20, 2025)

• TruSeq Stranded RNA Kit FAQs: differences in protocol with single and dual index adapters, and LS and HS workflows (January 20, 2025)

• TruSeq Stranded RNA Kit FAQs: input requirements (January 20, 2025)

• TruSeq Stranded RNA Kit FAQs: mRNA purification and rRNA removal (January 20, 2025)

• TruSeq Stranded RNA Kit FAQs: storage and freeze/thaw recommendations (January 20, 2025)

• Two Channel Chemistry and Imaging on the MiSeq i100 Series (January 20, 2025)

• Unlocking an account or resetting the password on Local Run Manager version 4 (January 20, 2025)

• Viral Surveillance Panel (VSP) probes and panel design frequently asked questions (FAQs) (January 20, 2025)

• Viral Surveillance Panel (VSP) reagents and workflow frequently asked questions (FAQs) (January 20, 2025)

• Viral Surveillance Panel (VSP) sequencing and analysis frequently asked questions (FAQs) (January 20, 2025)

• Wash Flow Cell Use Counter on NovaSeq X Series (January 20, 2025)

• What are the dimensions of the Infinium hybridization oven? (January 20, 2025)

• What is the Illumina Lysis Reagent Kit, and when should it be used? (January 20, 2025)

• What is the correct format for DRAGEN Array IDAT CSV Sample Sheet? (January 20, 2025)

• What is the correct order of addition of Infinium Amplification reagents? (January 20, 2025)

• When will Live %Demux information be available for a MiSeq i100 Series run? (January 20, 2025)

• Where to find the reference sequence used for phiX alignment? (January 20, 2025)

• Which BaseSpace apps and features use Illumina Connected Analytics (January 20, 2025)

• Which Nextera Index Kits come in plate or tube format? (January 20, 2025)

• Which TruSeq index kits come in plate or tube format? (January 20, 2025)

• Which computer operating systems are compatible with Illumina Experiment Manager (IEM)? (January 20, 2025)

• Anchor ligation best practices for Illumina Stranded mRNA and Total RNA Prep Ligation workflows (December 23, 2024)

• Bead clean up ratios used in the Illumina 16S demonstrated protocol (December 23, 2024)

• Best practices for handling magnetic beads used with Illumina RNA library preparation kits (December 23, 2024)

• Capture and wash best practices for Illumina RNA Prep with Enrichment (L) Tagmentation (December 23, 2024)

• DRAGEN TruSight Oncology 500 (TSO500) co install options (December 23, 2024)

• General best practices for Illumina RNA library preparation kits (December 23, 2024)

• How To Enable/Disable Illumina Proactive on iSeq 100 (December 23, 2024)

• How do stagger sequences or heterogeneity spacers introduce diversity? (December 23, 2024)

• How to assess rRNA depletion success before sequencing? (December 23, 2024)

• Illumina RNA Prep Tagmentation (L) with Enrichment General FAQs (December 23, 2024)

• Illumina RNA Prep Tagmentation (L) with Enrichment Input FAQs (December 23, 2024)

• Illumina RNA Prep Tagmentation (L) with Enrichment Protocol FAQs (December 23, 2024)

• Illumina Stranded mRNA Prep Ligation General FAQs (December 23, 2024)

• Illumina Stranded mRNA Prep Ligation Input FAQs (December 23, 2024)

• Illumina Stranded mRNA Prep Ligation Sequencing FAQs (December 23, 2024)

• Input FAQs for the Illumina TruSight RNA Pan Cancer library preparation kit (December 23, 2024)

• Input recommendations and considerations for COVIDSeq (RUO) library preparation (December 23, 2024)

• Is the Nextera TDE1 enzyme pre loaded with oligos? (December 23, 2024)

• Library quantification and QC for Illumina TruSight RNA Pan Cancer libraries (December 23, 2024)

• Protocol FAQs for Illumina Stranded Total RNA Prep Ligation with Ribo Zero Plus (December 23, 2024)

• RNA handling best practices for Illumina RNA library preparation kits (December 23, 2024)

• Reference images for NovaSeq 6000 (December 23, 2024)

• Reference images for the MiSeq (December 23, 2024)

• Ribo Zero Plus depletion best practices (December 23, 2024)

• TruSeq Small RNA Product and Support Pages (December 23, 2024)

• TruSeq Stranded mRNA Product and Support Pages (December 23, 2024)

• Types of small RNA and the need for a separate small RNA kit (December 23, 2024)

• What are the main differences between TruSeq Stranded and Illumina Stranded kits? (December 23, 2024)

• What volume of TruSeq RNA UD indexes should be used during TruSeq Stranded RNA library preparation? (December 23, 2024)

• Consumable catalog numbers for the MiSeq i100 Series (December 2, 2024)

• File paths on the MiSeq i100 Series (December 2, 2024)

• How to Export Logs on the MiSeq i100 Series (December 2, 2024)

• Tested Product Guidance for USB External Storage Devices on the MiSeq i100 Series (December 2, 2024)

• Troubleshooting Flow Cell Vacuum Failed to Engage on the NovaSeq X/X Plus (December 2, 2024)

• Troubleshooting Stalled Flow Rate Check on the MiSeq (December 2, 2024)