What's New

Articles published to Illumina Knowledge in the past 2 months:

• A quick visualization method to help determine the sex of given samples in a GenomeStudio Methylation project (February 20, 2025)

• Activating TeamViewer on the MiSeq i100 Series Instruments (February 20, 2025)

• AmpliSeq Custom DNA Panel product and support pages (February 20, 2025)

• AmpliSeq Custom RNA Panel product and support pages (February 20, 2025)

• AmpliSeq On Demand Panel Product and Support Pages (February 20, 2025)

• Are technical replicates needed for TruSeq Stranded RNA Seq experiments? (February 20, 2025)

• Best Practices for Nuclei Isolation with alternative nuclei isolation protocols for Illumina Single Cell (February 20, 2025)

• Best practices for cell preparation for Illumina Single Cell prep (February 20, 2025)

• Best practices for using Illumina Single Cell Nuclei Isolation Kits for Illumina Single Cell kits (February 20, 2025)

• COVIDSeq timing and logistics (February 20, 2025)

• Can Genotype Call (GTC) files be used as input for GenomeStudio? (February 20, 2025)

• Can additional users be added to the Linux interface on the NextSeq 1000/2000? (February 20, 2025)

• Can library preparation reagents be stored in a frost free freezer? (February 20, 2025)

• Can vortexing damage cells/nuclei when generating emulsions in the Illumina Single Cell workflow? (February 20, 2025)

• Differences between Illumina DNA Prep (M) Tagmentation (Nextera DNA Flex), Nextera DNA, and Nextera XT kits (February 20, 2025)

• Differences with TruSeq Stranded RNA LS and HS protocols and single and dual index adapters (February 20, 2025)

• Does DRAGEN Array have a Call Rate limit to estimate gender? (February 20, 2025)

• Effects of library quality and indexes on demultiplexing NovaSeq X Series data (February 20, 2025)

• End of Life Schedule for NextSeq 1000 / 2000 SBS Standard Consumables (February 20, 2025)

• Estimating Disk Space Utilization on the MiSeq i100 Series (February 20, 2025)

• Handling and disposal information for used reagents on the MiSeq i100 Series (February 20, 2025)

• How does the Illumina/Nextera tagmentation enzyme make sure that both p5 and p7 adapters are added? (February 20, 2025)

• How to Access the Linux Terminal on the MiSeq i100 Series Instruments (February 20, 2025)

• How to Configure Network Settings for the MiSeq i100 after First Time Setup (February 20, 2025)

• How to Find the Control Software Version on the NextSeq 500/550 (February 20, 2025)

• How to Power Cycle the MiSeq i100 Series (February 20, 2025)

• How to Restart File Transfer on the MiSeq i100 Series (February 20, 2025)

• How to Restart File Transfer on the NovaSeq X Series (February 20, 2025)

• How to Setup a Remote Connection to Illumina Run Manager on the MiSeq i100 Series Instruments (February 20, 2025)

• How to Setup the System Health Checks on the MiSeq i100 Series Instruments (February 20, 2025)

• How to Spike in PhiX for the MiSeq i100 Series (February 20, 2025)

• How to Troubleshoot Clustering Failures on the MiSeq i100 Series (February 20, 2025)

• How to Troubleshoot a "no base calls are available for its lane(s)" error in Local Run Manager (February 20, 2025)

• How to find a Safety Data Sheet (February 20, 2025)

• How to identify when an air filter was changed on the iSeq 100. (February 20, 2025)

• How to perform Linux OS updates on the NextSeq 1000/2000 (February 20, 2025)

• How to requeue analysis using only an index 2 (i5) read (February 20, 2025)

• How to retrieve the DRAGEN (FPGA) Serial Number for MiSeq i100 Series Systems (February 20, 2025)

• How to update the DRAGEN BIOS version to reduce v4 server fan noise (February 20, 2025)

• How to upload files to Illumina Kiteworks using command line tools (February 20, 2025)

• How to use the Log Bundler Lite application on NovaSeq X Series (February 20, 2025)

• Illumina Single Cell 3' RNA Preparation library sequencing recommendations and guidelines (February 20, 2025)

• Impact of RNA degradation on mRNA seq data (February 20, 2025)

• Indexed Sequencing workflows with 5' to 3' directionality (February 20, 2025)

• Information about the TruSeq index adapter plate fixture (February 20, 2025)

• Information and protocol for the Illumina 16S Metagenomics Sequencing Library Preparation workflow (February 20, 2025)

• Information and protocol for the Illumina Fungal Metagenomics Sequencing workflow (February 20, 2025)

• Instrument reference images for the MiSeq i100 Series (February 20, 2025)

• Instrument, flow cell, and reagent requirement FAQ for Illumina DNA PCR Free libraries (February 20, 2025)

• Introducing the Illumina NovaSeq X System (February 20, 2025)

• Is a sample sheet required to run DRAGEN Array? (February 20, 2025)

• Library storage FAQ for Illumina DNA PCR Free libraries (February 20, 2025)

• Link to the Sequencing Methods Explorer (February 20, 2025)

• Loading Concentration Optimization Recommendations for the MiSeq i100 Series (February 20, 2025)

• NextSeq 1000/2000 Control Software upgrade from 1.7.0 to 1.7.1 system integration error (February 20, 2025)

• Overview of the Fungal Metagenomic Sequencing Demonstrated Protocol (February 20, 2025)

• PhiX requirements for sequencing Illumina Stranded RNA libraries (February 20, 2025)

• Qualification/QC FAQ for Illumina DNA PCR Free libraries (February 20, 2025)

• Reagent Stability Guidelines for the MiSeq i100 Series Consumables (February 20, 2025)

• Recommended Network Security Allow List for the MiSeq i100 Series (February 20, 2025)

• Resources for getting started with Illumina Next Generation Sequencing (NGS) (February 20, 2025)

• Sample Sheet Import Errors on the NextSeq 1000/2000 (February 20, 2025)

• Secure communication protocols on Illumina systems (February 20, 2025)

• Sequencing Illumina Stranded Total RNA and mRNA Libraries on the MiSeq i100 Series (February 20, 2025)

• Sequencing TruSeq Small RNA libraries (February 20, 2025)

• Sequencing depth and read length considerations for TruSeq Stranded RNA libraries (February 20, 2025)

• Serial Number locations on Illumina sequencers (February 20, 2025)

• Size selection and clean up considerations for TruSeq DNA PCR Free library preparation (February 20, 2025)

• Troubleshooting "Additional instrument configuration is required" Issues on the MiSeq i100 Series (February 20, 2025)

• Troubleshooting "Component Failed" errors immediately after Pre Run Checks on the NovaSeq 6000 (February 20, 2025)

• Troubleshooting "Failed to communicate with RTA after 4 attempts" on the MiniSeq or NextSeq 500/550 (February 20, 2025)

• Troubleshooting "GenerateFASTQ 500 Internal Server Error" in Local Run Manager hosted on the iSeq 100 (February 20, 2025)

• Troubleshooting 'A configuration file cannot be assessed' Warnings during Pre Run Checks on the NovaSeq X Series (February 20, 2025)

• Troubleshooting 'An unexpected error occurred during sequencing' Errors on the MiSeq i100 Series (February 20, 2025)

• Troubleshooting 'EdgeOS You are offline' errors on the MiSeq i100 Series (February 20, 2025)

• Troubleshooting 'Failed to start First Time Setup' Errors on the MiSeq i100 Series Instruments (February 20, 2025)

• Troubleshooting 'One or more unknown settings found' Errors during Run Planning on the NovaSeq X Series (February 20, 2025)

• Troubleshooting 'Status code 523' Errors in Cloud Settings on the MiSeq i100 Series (February 20, 2025)

• Troubleshooting BIM SOUR 2 Handle Position Errors During NovaSeq 6000 System Checks (February 20, 2025)

• Troubleshooting Inaccurate Estimated Completion Time on the MiSeq i100 Series (February 20, 2025)

• Troubleshooting Index Dropouts or No Reads Identified for Single Sample on the MiSeq i100 Series (February 20, 2025)

• Troubleshooting Insufficient Space on External Storage on the MiSeq i100 Series Instruments. (February 20, 2025)

• Troubleshooting Internal Server Error in Cloud Settings on the MiSeq i100 Series (February 20, 2025)

• Troubleshooting Issues with Allocating Disk Space during Pre Run Checks on the NovaSeq X Series (February 20, 2025)

• Troubleshooting Leak Detection Errors on the MiSeq i100 Series (February 20, 2025)

• Troubleshooting Local Run Manager originally caused by an internal server error on NextSeq 500/550 (February 20, 2025)

• Troubleshooting Memory Metal Alloy (MMA) Motion Stalls on the MiSeq i100 Series (February 20, 2025)

• Troubleshooting Pressure Charging Failures on the MiSeq i100 Series (February 20, 2025)

• Troubleshooting Stalled Cloud Login Screen on the MiSeq i100 Series (February 20, 2025)

• Troubleshooting Stalled Secondary Analyses Onboard the MiSeq i100 Series. (February 20, 2025)

• Troubleshooting Unable to connect to external storage Errors on the MiSeq i100 Series (February 20, 2025)

• Troubleshooting elevated PhiX alignment in sequencing runs (February 20, 2025)

• TruSeq RNA v2 product and support page links (February 20, 2025)

• TruSeq Small RNA input best practices (February 20, 2025)

• TruSeq Stranded RNA kit storage and freeze/thaw recommendations (February 20, 2025)

• TruSeq Stranded RNA library preparation input requirements (February 20, 2025)

• Unlocking an account or resetting the password on Local Run Manager version 4 (February 20, 2025)

• Updated guidance for using new blue Infinium Staining Set (February 20, 2025)

• Viral Surveillance Panel (VSP) reagents and workflow frequently asked questions (FAQs) (February 20, 2025)

• Wash Flow Cell Use Counter on NovaSeq X Series (February 20, 2025)

• What are the dimensions of the Infinium hybridization oven? (February 20, 2025)

• What is the Illumina Lysis Reagent Kit, and when should it be used? (February 20, 2025)

• What is the correct format for DRAGEN Array IDAT CSV Sample Sheet? (February 20, 2025)

• What is the difference between a single and dual indexed library? (February 20, 2025)

• When will Live %Demux information be available for a MiSeq i100 Series run? (February 20, 2025)

• Where to find the reference sequence used for phiX alignment? (February 20, 2025)

• Which Nextera Index Kits come in plate or tube format? (February 20, 2025)

• Which TruSeq index kits come in plate or tube format? (February 20, 2025)

• Which computer operating systems are compatible with Illumina Experiment Manager (IEM)? (February 20, 2025)

• Why is NextSeq 1000/2000 control software update not available from the Software Update menu? (February 20, 2025)

• mRNA purification and rRNA removal information for TruSeq Stranded RNA workflows (February 20, 2025)

• Anchor ligation best practices for Illumina Stranded mRNA and Total RNA Prep Ligation workflows (December 23, 2024)

• Bead clean up ratios used in the Illumina 16S demonstrated protocol (December 23, 2024)

• Best practices for handling magnetic beads used with Illumina RNA library preparation kits (December 23, 2024)

• Capture and wash best practices for Illumina RNA Prep with Enrichment (L) Tagmentation (December 23, 2024)

• DRAGEN TruSight Oncology 500 (TSO500) co install options (December 23, 2024)

• General best practices for Illumina RNA library preparation kits (December 23, 2024)

• How To Enable/Disable Illumina Proactive on iSeq 100 (December 23, 2024)

• How do stagger sequences or heterogeneity spacers introduce diversity? (December 23, 2024)

• How to assess rRNA depletion success before sequencing? (December 23, 2024)

• Illumina RNA Prep Tagmentation (L) with Enrichment General FAQs (December 23, 2024)

• Illumina RNA Prep Tagmentation (L) with Enrichment Input FAQs (December 23, 2024)

• Illumina RNA Prep Tagmentation (L) with Enrichment Protocol FAQs (December 23, 2024)

• Illumina Stranded mRNA Prep Ligation General FAQs (December 23, 2024)

• Illumina Stranded mRNA Prep Ligation Input FAQs (December 23, 2024)

• Illumina Stranded mRNA Prep Ligation Sequencing FAQs (December 23, 2024)

• Input FAQs for the Illumina TruSight RNA Pan Cancer library preparation kit (December 23, 2024)

• Input recommendations and considerations for COVIDSeq (RUO) library preparation (December 23, 2024)

• Is the Nextera TDE1 enzyme pre loaded with oligos? (December 23, 2024)

• Library quantification and QC for Illumina TruSight RNA Pan Cancer libraries (December 23, 2024)

• Protocol FAQs for Illumina Stranded Total RNA Prep Ligation with Ribo Zero Plus (December 23, 2024)

• RNA handling best practices for Illumina RNA library preparation kits (December 23, 2024)

• Reference images for NovaSeq 6000 (December 23, 2024)

• Reference images for the MiSeq (December 23, 2024)

• Ribo Zero Plus depletion best practices (December 23, 2024)

• TruSeq Small RNA Product and Support Pages (December 23, 2024)

• TruSeq Stranded mRNA Product and Support Pages (December 23, 2024)

• Types of small RNA and the need for a separate small RNA kit (December 23, 2024)

• What are the main differences between TruSeq Stranded and Illumina Stranded kits? (December 23, 2024)

• What volume of TruSeq RNA UD indexes should be used during TruSeq Stranded RNA library preparation? (December 23, 2024)