我所购买的SBS试剂盒最多能够满足多少个循环的测序？

为上机测序提供足够量的SBS试剂是确保测序获得成功的重要因素之一。如果运行过程中试剂量不够，会导致荧光信号强度和测序质量值降低，甚至可能引起测序失败。双端测序在不同测序仪上有不同的操作流程，具体可以参照Indexed Sequencing Overview Guide。在某些仪器上运行双端index测序时，还需要额外七个循环的试剂做第二个index测序前的化学合成反应（仅合成不拍照），此时计算试剂盒所能支持的测序循环数时，一定要考虑到这七个循环也要被包含在总循环数内。
下面表格列举出了多种试剂盒的规格及每种试剂盒最多支持的循环数，请将在某些仪器上运行双端index测序时额外的7个循环考虑在内:
Reagent Type
Reagent Kit Size
Maximum Number of Cycles
Additional Cycles Needed for Dual Index?
Text
iSeq 100
i1 (v1 or v2)
300
322
No
MiniSeq
​Rapid Kit​
100
128
Yes - 7 cycles
High Output or
Mid Output
75
92
No
​
150
168
​
​
​
300
318
​
​
​
MiSeq
v2
(including Micro and Nano kits)
50
79
Yes - 7 cycles
300
329
​
​
​
500
529
​
​
​
v3
150
179
​
​
600
629
​
​
​
NextSeq 500/550
High Output or Mid Output
75
92
No
150
168
​
​
​
300
318
​
​
​
HiSeq 1000/1500/2000/2500
Rapid SBS v2
50
79
7 cycles required for paired-end flow cells
200
229
​
​
​
500
529
​
​
​
TruSeq SBS v3
50
58
​
​
200
209
​
​
​
HiSeq SBS v4
50
79
​
​
250
279
​
​
​
NextSeq 1000/2000
​
​
​
​
P1
100
138
No
​
300
338
​
​
​
600
638
​
​
​
P2
100
138
No
​
200
238
​
​
​
300
338*
​
​
​
600
638
​
​
​
P3
50
88
No
​
100
138
​
​
​
200
238
​
​
​
300
327*
​
​
​
HiSeq 3000/4000
N/A
50
79
7 cycles required for single-read flow cells
150
179
​
​
​
300
329
​
​
​
NovaSeq 6000
SP v1.0
100
130
Yes - 7 cycles
200
230
​
​
​
300
330
​
​
​
500
530
​
​
​
S1 and S2 v1.0
100
130
​
​
200
230
​
​
​
300
330
​
​
​
S4 v1.0
200
230
​
​
300
330
​
​
​
SP v1.5​
100
138
No
​
200
238
​
​
​
300
338
​
​
​
500
538
​
​
​
S1 and S2 v1.5​
100
138
​
​
200
238
​
​
​
300
338
​
​
​
S4 v1.5​
35
72
​
​
200
238
​
​
​
300
338
​
​
​
*The NextSeq 1000/2000 P1和P2试剂盒含有338个cycles 而 P3 v3 试剂盒含有327个cycles.
关于每种测序试剂盒的详细信息请参阅对应的试剂支持页面，不同测序试剂盒支持的最长读长请参阅Maximum read length for Illumina sequencing platforms​





For any feedback or questions regarding this article (Illumina Knowledge Article #7400), contact Illumina Technical Support [email protected].

Reagent Type	Reagent Kit Size	Maximum Number of Cycles	Additional Cycles Needed for Dual Index?	Text
iSeq 100	i1 (v1 or v2)	300	322	No
MiniSeq	Rapid Kit	100	128	Yes - 7 cycles
High Output or Mid Output	75	92	No
150	168
300	318
MiSeq	v2 (including Micro and Nano kits)	50	79	Yes - 7 cycles
300	329
500	529
v3	150	179
600	629
NextSeq 500/550	High Output or Mid Output	75	92	No
150	168
300	318
HiSeq 1000/1500/2000/2500	Rapid SBS v2	50	79	7 cycles required for paired-end flow cells
200	229
500	529
TruSeq SBS v3	50	58
200	209
HiSeq SBS v4	50	79
250	279
NextSeq 1000/2000
P1	100	138	No
300	338
600	638
P2	100	138	No
200	238
300	338*
600	638
P3	50	88	No
100	138
200	238
300	327*
HiSeq 3000/4000	N/A	50	79	7 cycles required for single-read flow cells
150	179
300	329
NovaSeq 6000	SP v1.0	100	130	Yes - 7 cycles
200	230
300	330
500	530
S1 and S2 v1.0	100	130
200	230
300	330
S4 v1.0	200	230
300	330
SP v1.5	100	138	No
200	238
300	338
500	538
S1 and S2 v1.5	100	138
200	238
300	338
S4 v1.5	35	72
200	238
300	338

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