Waste Volumes for MiSeq i100 Series Reagents

Background

The volume of waste produced by a MiSeq i100 Series sequencing run depends on the wet cartridge types as well as whether the instrument is set to automatically purge unused reagents when the run is complete. The flowcell type (e.g., 5M, 25M, etc.) does not affect the waste volumes.

The table below shows the expected approximate waste volumes when using the wet cartridge type specified in the table. The waste volumes depend on whether the Purge reagent cartridge after run checkbox is selected or not.

When the Purge reagent cartridge option is de-selected, the total waste volume can vary based on the total number of cycles performed in the run, with shorter runs generating reduced waste volume.

Wet Cartridge Type

Purge Enabled Waste Volume (mL)

Purge Disabled Waste Volume (mL)*

100

230

89-94

300

230

157-163

600

385

278-286

***Waste volumes will vary slightly depending on whether index cycles were performed or not.

For any feedback or questions regarding this article (Illumina Knowledge Article #9382), contact Illumina Technical Support techsupport@illumina.com.

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