Waste Volumes for MiSeq i100 Series Reagents

Background

The volume of waste produced by a MiSeq i100 Series sequencing run depends on the wet cartridge types as well as whether the instrument is set to automatically purge unused reagents when the run is complete. The flowcell type (e.g., 5M, 25M, etc.) does not affect the waste volumes.

The table below shows the expected approximate waste volumes when using the wet cartridge type specified in the table. The waste volumes depend on whether the Purge reagent cartridge after run checkbox is selected or not.

When the Purge reagent cartridge option is de-selected, the total waste volume can vary based on the total number of cycles performed in the run, with shorter runs generating reduced waste volume.

Wet Cartridge Type	Purge Enabled Waste Volume (mL)	Purge Disabled Waste Volume (mL)*
100	230	89-94
300	230	157-163
600	385	278-286

***Waste volumes will vary slightly depending on whether index cycles were performed or not.

Reference images for the respective volumes when Purge is Enabled and Disabled:

100 cycles Cartridge:

-Purge Enabled (230 mL)

-Purge Disabled (89-94 mL)

300 cycles Cartridge:

-Purge Enabled (230 mL)

-Purge Disabled (157-163 mL)

600 cycles Cartridge:

-Purge Enabled (385 mL)

-Purge Disabled (278-286 mL)

For any feedback or questions regarding this article (Illumina Knowledge Article #9382), contact Illumina Technical Support [email protected].