Reagent Stability Guidelines for the MiSeq i100 Series Consumables

Background

The MiSeq i100 Series consumables are shipped and stored at room temperature, so there are no thawing steps required when preparing a sequencing run. However, once the packaging has been opened, the reagent kit and associated consumables should be used within the time specified below for optimal performance.

MiSeq i100 Dry Cartridge

Keep the Dry Cartridge in the foil packaging until it is ready to be loaded. The Dry Cartridge must be used within 4 hours of opening the foil packaging.

MiSeq i100 Wet Cartridge

Keep the Wet Cartridge in the foil packaging until it is ready to be loaded. The Wet Cartridge must be used within 4 hours of opening the foil packaging.

Denatured Libraries (Libraries + KLD Buffer)

Store the denatured libraries on ice after mixing the diluted libraries with the KLD buffer. The denatured libraries are stable on ice or at 4° C for up to 6 hours.

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