# Illumina DNA Prep with Enrichment（原Nextera Flex for Enrichment）第三方panel要求

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Illumina DNA Prep with Enrichment（原Nextera Flex for Enrichment）是一个靶向富集流程，Illumina和第三方富集panels均可兼容。\
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第三方DNA探针Panel要求

如果将第三方带有生物素标记的DNA探针panels用于Illumina DNA Prep with Enrichment流程，富集panels需要满足以下标准：

* 探针长度为80 bp或120 bp
* 500到675,000个探针
* 单链或双链DNA探针
* 单个样本到12个样本混合后进行富集，探针总投入量≥ 3 pmol

使用DNA探针进行靶向富集时，参考 [Illumina DNA Prep with Enrichment参考指南](https://support.illumina.com/sequencing/sequencing_kits/illumina-dna-prep-with-enrichment/documentation.html)。\
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第三方RNA探针Panel要求

如果将第三方带有生物素标记的RNA探针panels用于Illumina DNA Prep with Enrichment流程，富集panels需要满足以下标准：

* 探针长度为120 nt
* 300,000到675,000个探针
* 单链探针
* 单个样本到12个样本混合后进行富集，探针总投入量≥ 3 pmol

使用RNA探针进行靶向富集时，参考 [Nextera Flex for Enrichment RNA探针示范Protocol](https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_nextera/nextera-flex-enrichment/nextera-flex-for-enrichment-rna-probes-demonstrated-protocol-1000000070581-01.pdf).

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将第三方探针panels用于Illumina DNA Prep with Enrichment可能需要额外的优化和验证，因为第三方探针panel尚未经过Illumina的验证。

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #7100), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000007100%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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