Guidelines for Sample Preparation for Infinium Genotyping Assay DNA input
Infinium Assay Sample Input Guidelines
Sample input guidelines:
Infinium Assay Format
DNA input amount
DNA input volume
DNA input concentration
Applicable step(s)
HD, HTS, LCG, XT
200 ng
4 μl
50 ng/μl
Amplify WGA
LCG Quad
400 ng
8 μl
50 ng/μl
Amplify WGA
GDA + ePGx
* 100 ng for WGA plate
100 ng for PGx plate
(200 ng total) | 5 μl each | 20 ng/μl | * Amplify WGA
Amplify PGx | | Infinium EX | 100 ng | 5 μl | 20 ng/μl | Make WGA | | Infinium EX PGx | * 100 ng for WGA plate
100 ng for PGx plate
(200 ng total) | 5 μl each | 20 ng/μl | * Make WGA
Make PGx |
Any standard DNA extraction method or kit that provides high quality genomic DNA is suitable for Infinium Arrays.
DNA quantity* Determine DNA concentration using a double stranded DNA specific fluorometric method (eg, Qubit, Quant-it, PicoGreen) for accurate measurement of double-stranded DNA.
DNA quantification results from UV spectrophotometric methods do not accurately reflect the concentration of double-stranded DNA and are not recommended.
Using the quantification values from the Bioanalyzer or Tapestation is not advised.
DNA quality* The minimum recommended fragment size is 2 kb.
Illumina does not provide DNA integrity (DIN) guidelines or cut-offs for DNA input into the Infinium assay. DIN values from a Tapestation or BioAnalyzer can be used to assess DNA quality, but are not required.
The presence of RNA in samples does not interfere with the Infinium assay.
UV spectrophotometry can be used to assess DNA purity and quality. Consider standard purity specifications:
Protein/RNA free: 260/280 ratio >1.85
Solvent free: 260/230 ratio >2.0
Phenol free: no peak at 270 nm
Recommendations for processing low quality DNA in the Infinium Assay
The Infinium FFPE QC Kit:
Uses a real-time PCR assay to evaluate the quality of prospective DNA samples.
Determines whether the DNA samples are suitable for the restoration step.
Is only compatible with human samples.
The Infinium HD FFPE DNA Restore Kit:
Restores degraded DNA to an amplifiable state that can be used as input for the Infinium assay.
Is compatible with samples from any species.
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