What is the maximum read lengths and total number of cycles for MiSeq kits?
Illumina platforms and sequencing kits allow for a variety of sequencing applications. To ensure the highest level of quality, Illumina supports reads up to a certain length depending on the sequencing platform and SBS kit version.
It is possible to choose a longer read length during run setup in Local Run Manager (LRM) or the instrument control software. However, when the read length exceeds the supported length, Illumina cannot guarantee the data quality.
The table below lists the maximum supported read lengths for the different MiSeq kits.
Reagent Type | Maximum Read Length | Maximum Index Length | Reagent Kit Size | Maximum Number of Total Cycles | Additional Cycles Needed for Dual Index? |
MiSeq v2 | 51 | Not restricted by software | 50 cycles | 79 | Yes (7 cycles) |
MiSeq v2 (Including Micro and Nano Kits) | 151* | Not restricted by software | 300 cycles | 329 | Yes (7 cycles) |
MiSeq v2 (Including Nano Kits) | 251 | Not restricted by software | 500 cycles | 529 | Yes (7 cycles) |
MiSeq v3 | 151 | Not restricted by software | 150 cycles | 179 | Yes (7 cycles) |
MiSeq v3 | 301 | Not restricted by software | 600 cycles | 629 | Yes (7 cycles) |
\*Note: if performing single read sequencing, can run up to 1x251 bp read length.
For any feedback or questions regarding this article (Illumina Knowledge Article #2595), contact Illumina Technical Support techsupport@illumina.com. |
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