What is the maximum read lengths and total number of cycles for MiSeq kits?

Illumina platforms and sequencing kits allow for a variety of sequencing applications. To ensure the highest level of quality, Illumina supports reads up to a certain length depending on the sequencing platform and SBS kit version.

It is possible to choose a longer read length during run setup in Local Run Manager (LRM) or the instrument control software. However, when the read length exceeds the supported length, Illumina cannot guarantee the data quality.

The table below lists the maximum supported read lengths for the different MiSeq kits.

Reagent Type	Maximum Read Length	Maximum Index Length	Reagent Kit Size	Maximum Number of Total Cycles	Additional Cycles Needed for Dual Index?
MiSeq v2	51	Not restricted by software	50 cycles	79	Yes (7 cycles)
MiSeq v2 (Including Micro and Nano Kits)	151*	Not restricted by software	300 cycles	329	Yes (7 cycles)
MiSeq v2 (Including Nano Kits)	251	Not restricted by software	500 cycles	529	Yes (7 cycles)
MiSeq v3	151	Not restricted by software	150 cycles	179	Yes (7 cycles)
MiSeq v3	301	Not restricted by software	600 cycles	629	Yes (7 cycles)

\*Note: if performing single read sequencing, can run up to 1x251 bp read length.

For any feedback or questions regarding this article (Illumina Knowledge Article #2595), contact Illumina Technical Support [e-mail].