# Does the Flex Lysis Reagent Kit require the purchase of additional Illumina reagents?

**Does the Illumina DNA Prep Saliva Lysis Protocol require the purchase of additional Illumina reagents beyond the Illumina Flex Lysis Reagent kit?**

If the Flex Lysis kit is to be used with one of the Illumina DNA Prep kit configurations that include the Illumina Purification Bead (IPB) reagent, additional Illumina reagents are not required for the Saliva Lysis protocol.

* The IPB reagent needed for DNA isolation is provided in sufficient quantity within the Illumina DNA Prep kits that contain them.
  * Kit configurations that include IPB are:\
    \- Illumina DNA Prep, (M) Tagmentation (24 Samples, IPB), catalog 20060060\
    \- Illumina DNA Prep, (M) Tagmentation (96 Samples, IPB), catalog 20060059
* The protocol requires user-supplied consumables that include Oragene DNA collection tubes (for saliva sample collection), a 96-well PCR plate, freshly prepared 80% ethanol, and nuclease-free water.

**Note**: See Appendix 2 of the [Illumina DNA Prep reference guide](https://support.illumina.com/downloads/illumina-dna-prep-reference-guide-1000000025416.html) for the Saliva Lysis protocol.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #7307), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000007307%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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