> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/library-preparation/dna-library-prep/library-preparation-dna-library-prep-faq-list/000007307.md).

# Does the Flex Lysis Kit require the purchase of additional Illumina reagents?

**Does the Illumina DNA Prep Saliva Lysis, Blood Lysis, or Dried Blood Spot Extraction Protocols require the purchase of additional Illumina reagents beyond the Illumina Flex Lysis Reagent kit?**

If using one of the Illumina DNA Prep kit configurations that include the Illumina Purification Bead (IPB) reagent, additional Illumina reagents are not required for Saliva Lysis. Blood Lysis or Dried Blood Spot Extraction protocols will require the Flex lysis kit, but no other additional Illumina reagents.

* The IPB reagent needed for DNA isolation is provided in sufficient quantity within the Illumina DNA Prep kits that contain them.
  * Kit configurations that include IPB are:\
    \- Illumina DNA Prep, (M) Tagmentation (24 Samples, IPB), catalog 20060060\
    \- Illumina DNA Prep, (M) Tagmentation (96 Samples, IPB), catalog 20060059
* The Saliva Lysis protocol requires user-supplied consumables that include Oragene DNA collection tubes (for saliva sample collection), a 96-well PCR plate, freshly prepared 80% ethanol, and nuclease-free water.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #7307), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000007307%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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