Does the Flex Lysis Reagent Kit require the purchase of additional Illumina reagents?

Does the Illumina DNA Prep Saliva Lysis Protocol require the purchase of additional Illumina reagents beyond the Illumina Flex Lysis Reagent kit?

If the Flex Lysis Reagent kit (cat 20018706) is to be used with one of the Illumina DNA Prep kit configurations that include the Illumina Purification Bead (IPB) reagent, additional Illumina reagents are not required for the Saliva Lysis protocol.

• The IPB reagent needed for DNA isolation is provided in sufficient quantity within the Illumina DNA Prep kits that contain them.

  • Kit configurations that include IPB are: - Illumina DNA Prep, (M) Tagmentation (24 Samples, IPB), catalog 20060060 - Illumina DNA Prep, (M) Tagmentation (96 Samples, IPB), catalog 20060059

• The protocol requires user-supplied consumables that include Oragene DNA collection tubes (for saliva sample collection), a 96-well PCR plate, freshly prepared 80% ethanol, and nuclease-free water.

Note: See Appendix 2 of the Illumina DNA Prep reference guide for the Saliva Lysis protocol.

Does the Nextera DNA Flex Dried Blood Spot Extraction demonstrated protocol require the purchase of additional Illumina reagents beyond the Illumina Flex Lysis Reagent kit?

If the Flex Lysis Reagent kit is to be used with the Illumina DNA Prep kit configurations that include the Illumina Purification Bead (IPB) reagent; additional Illumina reagents are not required for the Dried Blood Spot demonstrated protocol. Note that one Flex Lysis Reagent kit provides 800 µl of the BLB reagent; as 20 µl are needed per sample with the dried blood spot workflow, only 40 dried blood spot samples can be processed per Flex Lysis Reagent Kit.

• The IPB reagent needed for DNA isolation is provided in sufficient quantity within the Illumina DNA Prep kits that contain them.

  • Kit configurations that include IPB are: - Illumina DNA Prep, (M) Tagmentation (24 Samples, IPB), catalog 20060060 - Illumina DNA Prep, (M) Tagmentation (96 Samples, IPB), catalog 20060059

• The protocol requires user-supplied consumables that include Dried Blood Spot card (GE Healthcare, catalog # 10534320), a 96-well PCR plate, 1.5 mL tube, 80% ethanol, and nuclease-free water.

Note: IPB can be used in place of SPB (Sample Purification Beads) in the demonstrated protocol.

For any feedback or questions regarding this article (Illumina Knowledge Article #7307), contact Illumina Technical Support [e-mail].