How to obtain Unmethylated and Methylated signal in GenomeStudio for each CpG in MethylationEPIC Array
Last updated
Was this helpful?
Last updated
Was this helpful?
For Methylation arrays, the Beta value is calculated as: Signal B/(Signal A + Signal B + 100)
where Signal A = Unmethylated and Signal B = Methylated
To obtain Signal A (Unmethylated) and Signal B (Methylated) values in GenomeStudio 2011.1 software in the Methylation Module:
Under the Sample Methylation Profile table, select the "Column Chooser" icon
The "Column Chooser" window pops up. Under Hidden Subcolumns on the right side, highlight "Signal_A" and "Signal_B".
Then click the "<=Show" button so that these values appear under Displayed Subcolumns.
The "Signal_A" and "Signal_B" columns should now appear as a subcolumn under each sample. Each row (cg probe) will have a corresponding Signal_A and Signal_B for each sample. Intensity = Signal_A + Signal_B
External Links: Recorded Webinar:
For any feedback or questions regarding this article (Illumina Knowledge Article #6306), contact Illumina Technical Support .