Disk Management on the NextSeq 1000/2000

The NextSeq 1000/2000 local hard drive space must be managed via the Disk Management option in the control software.

The NextSeq 1000/2000 temporary folder is located at /usr/local/Illumina/runs/[runfolder] and is a complete local copy of the output folder.

  • Deleting the temporary folder manually outside of Disk Management can result in negative consequences.

A sequencing run requires ~200 GB (and up to 600 GB) of local hard drive space. A warning notification displays when space is low.

Use the following steps to delete completed runs and/or genomes to clear hard drive space, as needed.

  1. From the control software menu select Disk Management.

  • The Disk Management screen appears with a list of runs and reference genomes saved to the local hard drive.

  1. For the run to be deleted, select Delete Run

  • Deleting a run deletes the local run folder. The output folder, which is a copy of the run folder, is retained.

  1. In the dialog box, select Yes, Delete Run to confirm deleting the run.

  2. Repeat steps 2 and 3 for each run to be deleted.

  3. For deleting a genome, select Delete Genome.

  4. In the dialog box, select Yes, Delete Genome.

  5. Repeat steps 5 and 6 for each genome that needs to be deleted.

  6. When finished, close Disk Management and return to the Home screen.

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