Disk Management on the NextSeq 1000/2000

The NextSeq 1000/2000 local hard drive space must be managed via the Disk Management option in the control software.

The NextSeq 1000/2000 temporary folder is located at /usr/local/Illumina/runs/[runfolder] and is a complete local copy of the output folder.

• Deleting the temporary folder manually outside of Disk Management can result in negative consequences.

A sequencing run requires ~200 GB (and up to 600 GB) of local hard drive space.

A warning notification may appear when space is low.

Note: A Pre-Run Check warning will appear if any Operating System (OS) partition is over 70% full, though sequencing runs can proceed after this warning is acknowledged. If any partition reaches over 90% full, the Pre-Run Check prevents further sequencing runs from starting and space must be cleared.

Use the following steps to delete completed runs and/or genomes to clear hard drive space, as needed.

1. From the control software menu select Disk Management.

• The Disk Management screen appears with a list of runs and reference genomes saved to the local hard drive.

1. For the run to be deleted, select Delete Run

• Deleting a run deletes the local run folder. The output folder, which is a copy of the run folder, is retained.

1. In the dialog box, select Yes, Delete Run to confirm deleting the run.

2. Repeat steps 2 and 3 for each run to be deleted.

3. For deleting a genome, select Delete Genome.

4. In the dialog box, select Yes, Delete Genome.

5. Repeat steps 5 and 6 for each genome that needs to be deleted.

6. When finished, close Disk Management and return to the Home screen.

For any feedback or questions regarding this article (Illumina Knowledge Article #2445), contact Illumina Technical Support [email protected].