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Which sequencers use patterned vs. nonpatterned flow cells?

Patterned flow cells consist of a nanowell substrate with billions of ordered wells. Compared to nonpatterned flow cells, the uniform cluster sizes enable optimal spacing and increased cluster density. See Knowledge Article Calculating Percent Passing Filter for Patterned and Nonpatterned Flow Cells for more information.
Instruments that use patterned flow cells: NovaSeq 6000, NovaSeq X/X Plus, NextSeq 1000/2000, iSeq 100, HiSeq 3000/4000/X.
Instruments that use non-patterned flow cells: MiSeq, NextSeq 500/550, MiniSeq, HiSeq 1000/1500/2000/2500.
For any feedback or questions regarding this article (Illumina Knowledge Article #7786), contact Illumina Technical Support [email protected].
Last modified 12d ago
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