# Which sequencers use patterned vs. nonpatterned flow cells?

Patterned flow cells consist of a nanowell substrate with billions of ordered wells. Compared to nonpatterned flow cells, the uniform cluster sizes enable optimal spacing and increased cluster density. See Knowledge Article **Calculating Percent Passing Filter for Patterned and Nonpatterned Flow Cells** for more information.

Instruments that use **patterned flow cells**: NovaSeq 6000, NovaSeq X/X Plus, NextSeq 1000/2000, MiSeq i100, iSeq 100, HiSeq 3000/4000/X.

Instruments that use **non-patterned flow cells**: MiSeq, NextSeq 500/550, MiniSeq, HiSeq 1000/1500/2000/2500.

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