> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/instrumentation/general/instrumentation-general-faq-list/000006622.md).

# How to determine the appropriate kit configuration to use based on the data needed?

To determine the number of reads needed for the run (project), multiply the desired reads per sample by the number of samples in the pool. With the total number of reads, identify the kit and sequencer that fits the data needs using the sequencer specifications pages, listed below.

* [Specifications for the iSeq 100 System](https://www.illumina.com/systems/sequencing-platforms/iseq/specifications.html)
* [Specifications for the MiniSeq System](https://www.illumina.com/systems/sequencing-platforms/miniseq/specifications.html)
* [Specifications for the MiSeq System](https://www.illumina.com/systems/sequencing-platforms/miseq/specifications.html)
* [Specifications for the NextSeq 500/550 System](https://www.illumina.com/systems/sequencing-platforms/nextseq/specifications.html)
* [Specifications for the NextSeq 1000/2000 System](https://www.illumina.com/systems/sequencing-platforms/nextseq-1000-2000/specifications.html)
* [Specifications for the HiSeq 3000/4000 System](https://www.illumina.com/systems/sequencing-platforms/hiseq-3000-4000/specifications.html)
* [Specifications for the NovaSeq 6000 System](https://www.illumina.com/systems/sequencing-platforms/novaseq/specifications.html)
* [Specifications for the NovaSeq X/X Plus](https://www.illumina.com/systems/sequencing-platforms/novaseq-x-plus/specifications.html)

\
\
\ <br>

|                                                                                                                                                                                                                                                                                                                                                                        |
| :--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #6622), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000006622%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


---

# Agent Instructions
This documentation is published with GitBook. GitBook is the documentation platform designed so that both humans and AI agents can read, navigate, and reason over technical content effectively. Learn more at gitbook.com.

## Querying This Documentation
If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter:

```
GET https://knowledge.illumina.com/instrumentation/general/instrumentation-general-faq-list/000006622.md?ask=<question>
```

The question should be specific, self-contained, and written in natural language.
The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.
