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Troubleshooting mid run shutdowns or reboots on the MiSeq
Users may encounter a MiSeq with the screen black or the Windows log in screen while a run was in progress. These mid-run instrument shutdowns and/or reboots may result from environmental or instrument-related root causes. These may be different from Blue Screen errors - for more information regarding a blue screen error on the MiSeq, review the article Troubleshooting Windows OS Crashes or Blue Screen Errors on Illumina Instruments.
To identify if the instrument spontaneously rebooted or shutdown, determine what was present on the instrument screen at the time the issue was discovered. The instrument screen will be at the home log-in screen if the system rebooted, and will display a black screen if the system shut down without rebooting.
To troubleshoot an environmental power supply:
- 1.Verify no interruption to power within the lab or power outage occurred. This may require contacting local facilities teams.
- 2.Verify instrument is connected to an Uninterruptable Power Supply (UPS). A UPS will provide several minutes of extra power, but will not sustain the instrument through longer periods of power outage.
- 3.Verify that no other equipment or instruments in lab were impacted
- 4.Verify the power switch on the back of the instrument is toggled to ON.
If there were no unexpected interruptions to the power supply to the instrument and the power switch is toggled to the ON position, collect the following files and information from the instrument and contact Illumina Technical Support at [email protected] for further assistance.
- 1.Collect the Run cycle logs (located in D:\Illumina\MiSeq Temp[Run_Folder]\Logs). Include the InterOp folder, RunInfo.xml file, and RunParameters.xml file as well.
- 2.Collect the MiSeq Control Software Logs (located in D:\Illumina\MiSeq Control Software).
- 3.Collect the Windows Event Logs from the instrument. Windows Event logs
- 1.Search for Event Viewer in the Start menu and open Event Viewer.
- 2.Click on the down arrow to show the Windows Logs.
- 3.Select Application, which will load a long list of errors, information, and warnings.
- 4.Save this event log by right clicking on Save All Events Asâ€¦ on the far right and save as an event file (.evtx).
- 5.Once the Application logs are saved, save the Security and System logs the same way as the Application logs (see steps 3 and 4 above).
- 4.Provide a screenshot of the "Installed Products" Page in the Windows Control Panel.
- 1.To view Installed Products: Navigate to Control Panel > Programs and Features > Uninstall or Change a Program > Sort by "Installed On".
- 5.Provide a screenshot of the" Windows Updates" page.
- 1.Windows 10: Start Menu > Settings > Update and Security > Windows Update > Update History > open to view if any recently installed updates.
- 2.Windows 7: Start > Control Panel > Windows Update > "View update history" link.
Last modified 12d ago