How much PhiX to spike in for well balanced, base diverse libraries?

The PhiX sequencing control is not required for runs where the library pool is well-balanced in terms of base diversity, such as whole-genome and RNA-seq libraries; however, Illumina recommends spiking at least 1% PhiX into the library pool as a sequencing control and in order to obtain the error rate metric.

For any feedback or questions regarding this article (Illumina Knowledge Article #6972), contact Illumina Technical Support techsupport@illumina.com.

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