For the complete documentation index, see llms.txt. This page is also available as Markdown.

How much PhiX to spike in for well balanced, base diverse libraries?

The PhiX sequencing control is not required for runs where the library pool is well-balanced in terms of base diversity, such as whole-genome and RNA-seq libraries; however, Illumina recommends spiking at least 1% PhiX into the library pool as a sequencing control and in order to obtain the error rate metric.

For any feedback or questions regarding this article (Illumina Knowledge Article #6972), contact Illumina Technical Support techsupport@illumina.com.

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