# Best practices for pipette mixing PIPs and cells/nuclei in Illumina Single Cell Prep
During the Capture and Lysis portion of the Illumina Single Cell Prep workflow, users pipette mix their sample, RNase inhibitors, and PIPs before the addition of partitioning reagent and droplet formation.
It is important to avoid creating foam; mix the viscous cell:PIP mixture slowly and do not press the pipette to the second stop. If excessive foam forms, centrifuge the PIP tubes on a minifuge for \~5 seconds and repeat pipette mixing.
\
\
\
| |
| :--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------: |
| *For any feedback or questions regarding this article (Illumina Knowledge Article #9709), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000009709%20-%20Library%20Preparation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |
---
# Agent Instructions: Querying This Documentation
If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.
Perform an HTTP GET request on the current page URL with the `ask` query parameter:
```
GET https://knowledge.illumina.com/library-preparation/illumina-single-cell-rna-prep/library-preparation-illumina-single-cell-rna-prep-reference_material-list/000009709.md?ask=
```
The question should be specific, self-contained, and written in natural language.
The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.
Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.