Best practices for pipette mixing PIPs and cells/nuclei in Illumina Single Cell Prep

During the Capture and Lysis portion of the Illumina Single Cell Prep workflow, users pipette mix their sample, RNase inhibitors, and PIPs before the addition of partitioning reagent and droplet formation.

It is important to avoid creating foam; mix the viscous cell:PIP mixture slowly and do not press the pipette to the second stop. If excessive foam forms, centrifuge the PIP tubes on a minifuge for ~5 seconds and repeat pipette mixing.

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