Troubleshooting Failed to execute connectivity check input parameters on the NextSeq 1000/2000

The “Failed to execute connectivity check, input parameters are invalid" error can appear when there are network or internet connection issues with the selected output folder. This error occurs when selecting the output folder from Settings menu of the NextSeq 1000/2000 control software.

Potential Causes include:

  • Invalid Output folder.

  • BaseSpace Hosting Location is not selected.

Troubleshooting steps:

  1. Verify the output folder selected is a valid folder.

  • See Knowledge Article: Networking and output locations on the NextSeq 1000/2000.

  1. Select a Hosting Location from the Connectivity > Hosting Location Dropdown menu.

If unable to resolve the error, contact Illumina Technical Support for further guidance.

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