Illumina建库试剂盒和对应的index试剂盒

为了给客户提供更多的灵活性,illumina可以将建库试剂盒和index试剂盒分开单独提供,用户可以单独购买index试剂盒,这种形式可以提供比较多的index选择。本文档针对常见的建库试剂盒,列出了对应的多种index选择,以及推荐的index试剂盒。这有助于用户根据具体的项目来选择合适的index试剂盒。

2020年5月,illumina发布了对部分IDT for Illumina index所作的改变,新的名称和货号可以在这篇文档中查看。

包含了index试剂盒的建库试剂盒没有在本文档中列出。

如果要获得更多关于index试剂盒的信息,可以参考Illumina adapter portfolio 支持文档。

建库试剂盒Index试剂盒名称Index试剂盒货号新的Index试剂盒名称新的货号

TruSeq™ DNA Nano TruSeq™ PCR Free

TruSeq DNA Single Indexes Set A & B (12 Indexes, 24 Samples per set)

A: 20015960 B: 20015961

TruSeq DNA CD Indexes (96 Indexes, 96 Samples)

20015949

IDT for Illumina – TruSeq DNA UD Indexes (24 Indexes, 96 Samples)

20020590

IDT for Illumina – TruSeq DNA UD Indexes (96 Indexes, 96 Samples)

20022370

IDT for Illumina - TruSeq DNA UD Indexes v2 (96 Indexes, 96 Samples)

20040870

TruSeq Stranded Total RNA TruSeq Stranded mRNA

TruSeq RNA Single Indexes Set A & B (12 Indexes, 48 Samples per set)

A: 20020492 B: 20020493

TruSeq RNA CD Index Plate (96 Indexes, 96 Samples)

20019792

IDT for Illumina – TruSeq RNA UD Indexes (24 Indexes, 96 Samples)

20020591

IDT for Illumina – TruSeq RNA UD Indexes (96 Indexes, 96 Samples)

20022371

IDT for Illumina - TruSeq RNA UD Indexes v2 (96 Indexes, 96 Samples)

20040871

Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Illumina Stranded mRNA Prep, Ligation

IDT for Illumina RNA UD Indexes Set A-D, Ligation (96 Indexes, 96 Samples per set)

A: 20040553 B: 20040554 C: 20040555* D: 20040556*

Illumina RNA Prep with Enrichment, (L) Tagmentation

IDT for Illumina - DNA/RNA UD Indexes Set A-D, Tagmentation (96 Indexes, 96 Samples per set)

A: 20027213 B: 20027214 C: 20042666 D: 20042667

TruSeq Targeted RNA Expression

TruSeq Targeted RNA Index Kit (48 Indexes, 48 Samples)

RT-401-1001

TruSeq Targeted RNA Index Kit A-D (96 Indexes, 96 Samples per set)

A: RT‑402‑1001 B: RT‑402‑1002 C: RT‑ D: RT‑402‑1004

Illumina DNA PCR-Free Prep, Tagmentation

IDT for Illumina Nextera DNA UD Indexes Set v1 A-B (96 Indexes, 96 Samples per set) Set v2 C-D

A: 20027213 B: 20027214 C: 20042666 D: 20042667

IDT for Illumina - DNA/RNA UD Indexes Set A-D, Tagmentation (96 Indexes, 96 Samples per set)

A: 20027213 B: 20027214 C: 20042666 D: 20042667

Illumina DNA Prep, (M) Tagmentation [Formerly Nextera DNA Flex]

IDT for Illumina Nextera DNA UD Indexes Set A-D (96 Indexes, 96 Samples per set)

A: 20027213 B: 20027214 C: 20027215 D: 20027216

IDT for Illumina - DNA/RNA UD Indexes Set A-D, Tagmentation (96 Indexes, 96 Samples per set)

A: 20027213 B: 20027214 C: 20042666 D: 20042667

Nextera DNA CD Indexes (24 Indexes, 24 Samples)

20018707

Nextera DNA CD Indexes (96 Indexes, 96 Samples)

20018708

Illumina DNA Prep with Enrichment, (S) Tagmentation [Formerly Nextera DNA Flex Pre-Enrichment Library Prep and Enrichment Reagents]

IDT for Illumina Nextera DNA UD Indexes Set A-D (96 Indexes, 96 Samples per set)

A: 20027213 B: 20027214 C: 20027215 D: 20027216

IDT for Illumina - DNA/RNA UD Indexes Set A-D, Tagmentation (96 Indexes, 96 Samples per set)

A: 20027213 B: 20027214 C: 20042666 D: 20042667

IDT for Illumina Nextera DNA UD Indexes Sets A-D (384 Indexes, 384 Samples)

20027217

Nextera XT DNA

IDT for Illumina Nextera DNA UD Indexes Set A-D (96 Indexes, 96 Samples per set)

A: 20027213 B: 20027214 C: 20027215 D: 20027216

IDT for Illumina - DNA/RNA UD Indexes Set A-D, Tagmentation (96 Indexes, 96 Samples per set)

A: 20027213 B: 20027214 C: 20042666 D: 20042667

Nextera XT Index Kit v2 Set A-D (96 Indexes, 384 Samples per set)

A: FC‑131‑2001 B: FC‑131‑2002 C: FC‑131‑2003 D: FC‑131‑2004

Nextera XT Index Kit (24 Indexes, 96 Samples)

FC-131-1001

AmpliSeq for Illumina library prep PLUS kits

AmpliSeq UD Indexes for Illumina (24 Indexes, 24 Samples)

20019104

AmpliSeq CD Indexes Set A-D for Illumina (96 Indexes, 96 Samples per set)

A: 20019105 B: 20019106 C: 20019107 D: 20019167

AmpliSeq CD Indexes Sets A-D for Illumina (384 Indexes, 384 Samples)

20031676

AmpliSeq CD Indexes Large Volume for Illumina (96 Indexes, 96 Samples)

20019108

* 尚未发布

For any feedback or questions regarding this article (Illumina Knowledge Article #7314), contact Illumina Technical Support techsupport@illumina.com.

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