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How to change a run folder name on the NextSeq 500/550

The following are instructions for changing the NextSeq 500/550 run folder name:

  1. Close the NextSeq 500/550 Control Software (NCS).

  2. Navigate to C:\ProgramData\Illumina\NextSeqControlSoftware\InstrumentShell.NextSeqControlSoftware.Options.cfg

  3. Create a backup copy of this config file. For example, name the backup copy InstrumentShell.NextSeqControlSoftware.Options.cfg.BAK

  4. In the original configuration file (ie, InstrumentShell.NextSeqControlSoftware.Options.cfg) edit the following line to update the instrument name, add the experiment name, etc.:

  1. Launch NCS.

An example of adding an experiment name is as follows: {YY}{MM}{DD}_{SERIAL}_{RUN}__A{FCSERIAL}_{EXPERIMENT}/RunIDTemplate>

Note: The experiment name is pulled from the sample sheet. As such, this will not work for runs set up in manual mode.

For any feedback or questions regarding this article (Illumina Knowledge Article #2087), contact Illumina Technical Support techsupport@illumina.com.

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