Links
Comment on page

Troubleshooting Camera exception, error= 360: Failed to perform sBCa on the MiSeq

The "Camera exception, error=-360: Failed to perform sBCa_LibraryReinitialize(60000) call." error during a MiSeq sequencing run could indicate a transient communication issue between the hardware and software.
Troubleshooting steps:
  1. 1.
    E-mail Illumina Technical Support at [email protected] with the information from the section below titled Information to Provide to Tech Support.
  2. 2.
    Complete the post-run wash.
  3. 3.
    Power cycle the MiSeq (note that if a run is in progress, it will not be able to be resumed).
    1. 1.
      Close MiSeq Control Software shut down Windows, and turn off the instrument using the power switch on the back panel.
    2. 2.
      Leave the instrument off for 5 minutes to allow all parts to completely power down.
    3. 3.
      Turn on the instrument using the power switch on the back panel. The control software will initialize on its own once Windows fully loads.
  4. 4.
    If the control software fails to initialize, contact Illumina Technical Support.
  5. 5.
    If the instrument initializes without error, proceed with the next run.
Information to provide to Tech Support:
  1. 1.
    System uptime: This can be seen from Task Manager - Performance tab.
  2. 2.
    Software uptime: Available on the 'MiSeq Control Software' About screen (the time restarts when software is launched).
  3. 3.
    MiSeq Control Software (MCS) version: Available on the 'MiSeq Control Software' About screen.
  4. 4.
    FPGA version: Available on the 'MiSeq Control Software' About screen.
  5. 5.
    MiSeq Control Software logs: D:\Illumina\MiSeq Control Software\Logs
  6. 6.
    Run cycle logs, if applicable: D:\Illumina\MiSeqAnalysis[run folder]\Logs folder OR D:\Illumina\MiSeqTemp[run folder]\Logs folder
  7. 7.
    Instrument usage rate: On average, how many runs were performed in the last month?
  8. 8.
    Run type, if applicable, when the error happened: Chemistry version and length of the run.
  • example: v2 500 cycle kit, 251 x 8 x 8 x 251
For any feedback or questions regarding this article (Illumina Knowledge Article #7301), contact Illumina Technical Support [email protected].
Last modified 12d ago
© 2023 Illumina, Inc. All rights reserved. All trademarks are the property of Illumina, Inc. or their respective owners. Trademark information: illumina.com/company/legal.html. Privacy policy: illumina.com/company/legal/privacy.html