> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/instrumentation/nextseq-500-550/instrumentation-nextseq-500-550-troubleshooting-list/000002103.md).
# Troubleshooting 'Lane Pump not initialized' fluidics related error on the NextSeq 500/550
This error indicates there is an issue with the fluidics system and it can appear at anytime (eg, during initialization, washes, pre-checks, during runs). This can be a communication error between the hardware and software, or an issue with the syringe pump itself.
**Error text:**
"Error in Fluidics.FlowCellFluidics1.LanePump. Device not initialized. Command = 'Q', reply = '7'"
**Troubleshooting steps:**
1. Power cycle\\\* the NextSeq 500/550.2. If the instrument initializes successfully, complete a Quick Wash.\\\*3. If the wash completes without errors, setup the next run on the instrument.4. If the error persists after initialization, contact [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/united-states/technical-support) for additional guidance
For further information, search for Knowledge Base articles **How to Perform a NextSeq 500/550 Power Cycle** and **How to perform washes on the NextSeq 500/550.**
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| *For any feedback or questions regarding this article (Illumina Knowledge Article #2103), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000002103%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |
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