# Troubleshooting 'Lane Pump not initialized' fluidics related error on the NextSeq 500/550 This error indicates there is an issue with the fluidics system and it can appear at anytime (eg, during initialization, washes, pre-checks, during runs). This can be a communication error between the hardware and software, or an issue with the syringe pump itself. **Error text:** "Error in Fluidics.FlowCellFluidics1.LanePump. Device not initialized. Command = 'Q', reply = '7'" ![](https://761066130-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FGM9W2DuBTgEXv1ClCm8H%2Fuploads%2Fgit-blob-3fe1686f77616c4a00aff0a2e17602eb34d38654%2Fimage1.jpg?alt=media\&token=b5c87810-5b19-4090-8e66-510edef1dbd1) **Troubleshooting steps:** 1. Power cycle\\\* the NextSeq 500/550.2. If the instrument initializes successfully, complete a Quick Wash.\\\*3. If the wash completes without errors, setup the next run on the instrument.4. If the error persists after initialization, contact [Illumina Technical Support](https://www.illumina.com/company/contact-us.html#/united-states/technical-support) for additional guidance For further information, search for Knowledge Base articles **How to Perform a NextSeq 500/550 Power Cycle** and **How to perform washes on the NextSeq 500/550.** \ \ \
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