Troubleshooting 'Lane Pump not initialized' fluidics related error on the NextSeq 500/550

This error indicates there is an issue with the fluidics system and it can appear at anytime (eg, during initialization, washes, pre-checks, during runs). This can be a communication error between the hardware and software, or an issue with the syringe pump itself.

Error text:

"Error in Fluidics.FlowCellFluidics1.LanePump. Device not initialized. Command = 'Q', reply = '7'"

Troubleshooting steps:

1. Power cycle\* the NextSeq 500/550.2. If the instrument initializes successfully, complete a Quick Wash.\*3. If the wash completes without errors, setup the next run on the instrument.4. If the error persists after initialization, contact Illumina Technical Support for additional guidance

For further information, search for Knowledge Base articles How to Perform a NextSeq 500/550 Power Cycle and How to perform washes on the NextSeq 500/550.

For any feedback or questions regarding this article (Illumina Knowledge Article #2103), contact Illumina Technical Support [e-mail].