# Ribo Zero Plus depletion best practices

**Ribo-Zero Plus Depletion Best Practices**

* Prepare fresh 80% EtOH daily for bead cleanup steps
* Bring RNAClean XP beads to room temperature for at least 30 minutes
* Make sure thermal cycler lid is preheated
* Thermal cycler must be capable of 0.1°C/second ramping

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