How to Plot %Occupied by %Passing Filter to optimize loading for the NovaSeq X Series, NovaSeq 6000, MiSeq i100 Series, and iSeq 100 Video
The %Occupied by %Pass Filter plot allows users to optimize the loading concentration for NovaSeq 6000 and iSeq 100. The %Occupied and %Pass Filter metrics can be plotted in Sequencing Analysis Viewer (SAV) to determine if a run was underloaded, optimally loaded, or overloaded. This tutorial covers how to create the plot and how to interpret it.
Video length: 1:57 min
This information is also available in written form. For further information, search for Knowledge Base article Plotting %Occupied by %Pass Filter to optimize loading concentration for NovaSeq 6000 and iSeq 100 platforms.
For any feedback or questions regarding this article (Illumina Knowledge Article #5858), contact Illumina Technical Support techsupport@illumina.com.
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