# Plotting %Occupied by %Pass Filter to optimize loading for the NovaSeq 6000 and iSeq 100 Video

The %Occupied by %Pass Filter plot allows users to optimize the loading concentration for NovaSeq 6000 and iSeq 100. The %Occupied and %Pass Filter metrics can be plotted in Sequencing Analysis Viewer (SAV) to determine if a run was underloaded, optimally loaded, or overloaded. This tutorial covers how to create the plot and how to interpret it.

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{% embed url="<https://www.youtube.com/watch?v=xuLpI4pZ13I&ab_channel=Illumina>" %}

Video length: 1:57 min

This information is also available in written form. For further information, search for Knowledge Base article **Plotting %Occupied by %Pass Filter to optimize loading concentration for NovaSeq 6000 and iSeq 100 platforms**.

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