> For the complete documentation index, see [llms.txt](https://knowledge.illumina.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://knowledge.illumina.com/instrumentation/general/instrumentation-general-reference_material-list/000005858.md).

# How to Plot %Occupied by %Passing Filter to optimize loading for the NovaSeq X Series, NovaSeq 6000, MiSeq i100 Series, and iSeq 100 Video

The %Occupied by %Pass Filter plot allows users to optimize the loading concentration for NovaSeq 6000 and iSeq 100. The %Occupied and %Pass Filter metrics can be plotted in Sequencing Analysis Viewer (SAV) to determine if a run was underloaded, optimally loaded, or overloaded. This tutorial covers how to create the plot and how to interpret it.

{% embed url="<https://www.youtube.com/watch?v=xuLpI4pZ13I&ab_channel=Illumina>" %}

Video length: 1:57 min

This information is also available in written form. For further information, search for Knowledge Base article **Plotting %Occupied by %Pass Filter to optimize loading concentration for NovaSeq 6000 and iSeq 100 platforms**.

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| *For any feedback or questions regarding this article (Illumina Knowledge Article #5858), contact Illumina Technical Support* [*techsupport@illumina.com*](mailto:techsupport@illumina.com?subject=Question%2FFeedback%20Regarding%20Illumina%20Knowledge%20Article%20#000005858%20-%20Instrumentation%20\&body=Dear%20Illumina%20Technical%20Support,%0D%0A%0D%0A)*.* |


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