# Approximate sizes of sequencing run output folders **Background** The run folder sizes listed in this bulletin should be used only as an approximation. Folder size will vary depending on the raw cluster density and the number of clusters passing filter. Folder sizes are based on runs with optimal cluster densities for each platform and dual indexing. For runs on nonpatterned flow cells, the size approximations are based on runs with pass filter greater than 80%. Additional run folder size considerations: * iSeq 100 folder sizes include FASTQ files generated by the Local Run Manager FASTQ workflow. * MiniSeq, NextSeq 500/550 (NextSeq Control Software v4.0 and higher) folder sizes include FASTQ files generated by the Local Run Manager FASTQ workflow. * MiSeq folder sizes include FASTQ files generated by the MiSeq Reporter FASTQ workflow and MiSeq (MiSeq Control Software 3.1 and higher) Local Run Manager FASTQ workflow. * NextSeq 1000/2000 folder sizes include local analysis output, which can add another 25-120+ GB depending on workflow. * NovaSeq 6000 folders do not include FASTQ files as part of the total output folder size. Illumina recommends storing the output folder for iSeq 100, NextSeq 500/550, and NextSeq 1000/2000 runs on a network location. NovaSeq 6000 and NovaSeq X Series Control Software requires the output folder be stored on a network location. **iSeq 100** | Read Length | Output Folder Size in GB | | ----------- | ------------------------ | | 2 x 150 | 1.5 - 2.5 | **MiniSeq** Mid Output | Read Length | Output Folder Size in GB | | ----------- | ------------------------ | | 2 x 150 | 2.3 - 2.6 | High Output | Read Length | Output Folder Size in GB | | ----------- | ------------------------ | | 2 x 150 | 7.0 - 8.0 | | 2 x 75 | 6.0 - 8.0 | | 1 x 75 | 2.0 - 4.0 | Rapid | Read Length | Output Folder Size in GB | | ----------- | ------------------------ | | 1 x 100 | 2 | **MiSeq** v3 chemistry | Read Length | Output Folder Size in GB | | ----------- | ------------------------ | | 2 x 300 | 22 - 26 | | 2 x 75 | 19 - 22 | v2 chemistry | Read Length | Reagent Kit Configuration | Output Folder Size in GB | | ----------- | ------------------------- | ------------------------ | | 2 x 250 | Standard | 22 - 24 | | 2 x 250 | Nano | 1.6 - 1.8 | | 2 x 150 | Standard | 16 - 18 | | 2 x 150 | Micro | 3.0 - 5.0 | | 2 x 150 | Nano | 0.8 - 1.2 | | 2 x 25 | Standard | 14 - 16 | **MiSeq i100 Series** See Knowledge Article [Estimating Disk Space Utilization on the MiSeq i100 Series](https://knowledge.illumina.com/instrumentation/miseq-i100-series/instrumentation-miseq-i100-series-reference_material-list/000009356) **NextSeq 500/550** Mid Output | Read Length | Output Folder Size in GB | | ----------- | ------------------------ | | 2 x 150 | 62 - 66 | | 2 x 75 | 39 - 46 | High Output | Read Length | Output Folder Size in GB | | ----------- | ------------------------ | | 2 x 150 | 160 - 170 | | 2 x 75 | 92 - 97 | | 1 x 75 | 50 - 78 | **NextSeq 1000/2000 (Standard and XLEAP runs are expected to have similar sizes)** Note: Information for P4 runs is not available yet. P1 | Read Length | Output Folder Size in GB | | ----------- | ------------------------ | | 2 x 300 | 85-95 | | 2 x 150 | 50 - 55 | | 2 x 101 | 40 - 45 | | 2 x 75 | 35 - 40 | P2 | Read Length | Output Folder Size in GB | | ----------- | ------------------------ | | 2 x 300 | 140-160 | | 2 x 150 | 89 - 99 | | 2 x 101 | 65 - 66 | | 2 x 75 | 44 - 55 | P3 | Read Length | Output Folder Size in GB | | ----------- | ------------------------ | | 2 x 150 | 165 - 170 | | 2 x 101 | 103 - 126 | P4 XLEAP | Read Length | Output Folder Size in GB | | ----------- | ------------------------ | | 2 x 150 | 170 - 180 | | 2 x 100 | 100 - 130 | | 2 x 50 | 70 - 80 | | 1 x 50 | 40 - 50 | **NovaSeq 6000** SP | Read Length | Output Folder Size in GB | | ----------- | ------------------------ | | 1 x 36 | 20 | | 2 x 150 | 185 | | 2 x 250 | 400 | S1 | Read Length | Output Folder Size in GB | | ----------- | ------------------------ | | 2 x 150 | 370 | S2 | Read Length | Output Folder Size in GB | | ----------- | ------------------------ | | 2 x 150 | 730 | S4 | Read Length | Output Folder Size in GB | | ----------- | ------------------------ | | 1 x 36 | 240 | | 2 x 150 | 2190 | The output folder size in the above tables is reported in Gigabytes (GB). **NovaSeq X/X Plus** Values listed below are PER FLOW cell in Terabytes (TB). 1.5B | Read Length | cBCL Only in TB | BCL Convert (GZIP) in TB | BCL Convert (ORA) in TB | | ----------- | --------------- | ------------------------ | ----------------------- | | 2 x 50 | 0.054 - 0.091 | 0.132 - 0.221 | 0.086 - 0.143 | | 2 x 100 | 0.106 - 0.181 | 0.265 - 0.442 | 0.173 - 0.286 | | 2 x 150 | 0.163 - 0.272 | 0.397- 0.662 | 0.259 - 0.429 | 10B | Read Length | cBCL Only in TB | BCL Convert (GZIP) in TB | BCL Convert (ORA) in TB | | ----------- | --------------- | ------------------------ | ----------------------- | | 2 x 50 | 0.34 - 0.57 | 0.83 - 1.38 | 0.54 - 0.89 | | 2 x 100 | 0.68 - 1.13 | 1.65 - 2.76 | 1.08 - 1.79 | | 2 x 150 | 1.02 - 1.70 | 2.48 - 4.14 | 1.62 - 2.68 | 25B | Read Length | cBCL Only in TB | BCL Convert (GZIP) in TB | BCL Convert (ORA) in TB | | ----------- | --------------- | ------------------------ | ----------------------- | | 2 x 50 | 0.85 - 1.42 | 2.07 - 3.45 | 1.35 - 2.23 | | 2 x 100 | 1.70 - 2.83 | 4.13 - 6.90 | 2.70 - 4.47 | | 2 x 150 | 2.55 - 4.25 | 6.200 - 10.35 | 4.05 - 6.70 | DRAGEN Analysis files can ADD the following additional data in terabytes (TB) to the expected Output folder size based on Analysis options selected. Approximate file sizes are based on a single flow cell, 2x150 cycle sequencing run. | Flow Cell Type | BAM (TB) | CRAM (TB) | GVCF+VCF (TB) | | -------------- | -------- | --------- | ------------- | | 1.5B | 0.4 | 0.1 | <0.1 | | 10B | 2.5 | 1.0 | 0.2 | | 25B | 6.3 | 2.5 | 0.5 | \ \ \
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