Approximate sizes of sequencing run output folders
The run folder sizes listed in this bulletin should be used only as an approximation. Folder size will vary depending on the raw cluster density and the number of clusters passing filter. Folder sizes are based on runs with optimal cluster densities for each platform and dual indexing. For runs on nonpatterned flow cells, the size approximations are based on runs with pass filter greater than 80%.
Additional run folder size considerations:
iSeq 100 folder sizes include FASTQ files generated by the Local Run Manager FASTQ workflow.
MiniSeq, NextSeq 500/550 (NextSeq Control Software v4.0 and higher) folder sizes include FASTQ files generated by the Local Run Manager FASTQ workflow.
MiSeq folder sizes include FASTQ files generated by the MiSeq Reporter FASTQ workflow and MiSeq (MiSeq Control Software 3.1 and higher) Local Run Manager FASTQ workflow.
NextSeq 1000/2000 folder sizes include local analysis output, which can add another 25-120+ GB depending on workflow.
HiSeq 1500/2500, HiSeq 3000/4000, HiSeq X, and NovaSeq 6000 folders do not include FASTQ files as part of the total output folder size.
Folder sizes for HiSeq 1500/2500, HiSeq 3000/4000, HiSeq X, and NovaSeq 6000 are representative of a single flow cell.
Illumina recommends storing the output folder for iSeq 100, NextSeq 500/550, NextSeq 1000/2000, HiSeq 1500/2500, HiSeq 3000/4000, and HiSeq X runs on a network location. NovaSeq Control Software requires the output folder to be stored on a network location.
iSeq 100
Read Length
Output Folder Size in GB
2 x 150
1.5 - 2.5
MiniSeq
Mid Output
Read Length
Output Folder Size in GB
2 x 150
2.3 - 2.6
High Output
Read Length
Output Folder Size in GB
2 x 150
7.0 - 8.0
2 x 75
6.0 - 8.0
1 x 75
2.0 - 4.0
Rapid
Read Length
Output Folder Size in GB
1 x 100
2
MiSeq
v3 chemistry
Read Length
Output Folder Size in GB
2 x 300
22 - 26
2 x 75
19 - 22
v2 chemistry
Read Length
Reagent Kit Configuration
Output Folder Size in GB
2 x 250
Standard
22 - 24
2 x 250
Nano
1.6 - 1.8
2 x 150
Standard
16 - 18
2 x 150
Micro
3.0 - 5.0
2 x 150
Nano
0.8 - 1.2
2 x 25
Standard
14 - 16
NextSeq 500/550
Mid Output
Read Length
Output Folder Size in GB
2 x 150
62 - 66
2 x 75
39 - 46
High Output
Read Length
Output Folder Size in GB
2 x 150
160 - 170
2 x 75
92 - 97
1 x 75
50 - 78
NextSeq 1000/2000
P1
Read Length
Output Folder Size in GB
2 x 300
85-95
2 x 150
50 - 55
2 x 101
40 - 45
2 x 75
35 - 40
P2
Read Length
Output Folder Size in GB
2 x 300
140-160
2 x 150
89 - 99
2 x 101
65 - 66
2 x 75
44 - 55
P3
Read Length
Output Folder Size in GB
2 x 150
165 - 170
2 x 101
103 - 126
NovaSeq 6000
SP
Read Length
Output Folder Size in GB
1 x 36
20
2 x 150
185
2 x 250
400
S1
Read Length
Output Folder Size in GB
2 x 150
370
S2
Read Length
Output Folder Size in GB
2 x 150
730
S4
Read Length
Output Folder Size in GB
1 x 36
240
2 x 150
2190
NovaSeq X/X Plus
1.5B
Read Length
cBCL Only in GB
BCL Convert (GZIP) in GB
BCL Convert (ORA) in GB
2x50
54 - 91
132 - 221
86 - 143
2x100
106 - 181
265 - 442
173 - 286
2x150
163 - 272
397-662
259 - 429
10B
Read Length
cBCL Only in GB
BCL Convert (GZIP) in GB
BCL Convert (ORA) in GB
2x50
340 - 570
830 - 1380
540 - 890
2x100
680 - 1130
1650 - 2760
1080 - 1790
2x150
1020 - 1700
2480 - 4140
1620 - 2680
25B
Read Length
cBCL Only in GB
BCL Convert (GZIP) in GB
BCL Convert (ORA) in GB
2x150
2550 - 4250
6200 - 10350
4050 - 6700
The output folder size in these tables is reported in Gigabytes (GB).
For any feedback or questions regarding this article (Illumina Knowledge Article #1508), contact Illumina Technical Support techsupport@illumina.com.
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