Approximate sizes of sequencing run output folders
Background
The run folder sizes listed in this bulletin should be used only as an approximation. Folder size will vary depending on the raw cluster density and the number of clusters passing filter. Folder sizes are based on runs with optimal cluster densities for each platform and dual indexing. For runs on nonpatterned flow cells, the size approximations are based on runs with pass filter greater than 80%.
Additional run folder size considerations:
iSeq 100 folder sizes include FASTQ files generated by the Local Run Manager FASTQ workflow.
MiniSeq, NextSeq 500/550 (NextSeq Control Software v4.0 and higher) folder sizes include FASTQ files generated by the Local Run Manager FASTQ workflow.
MiSeq folder sizes include FASTQ files generated by the MiSeq Reporter FASTQ workflow and MiSeq (MiSeq Control Software 3.1 and higher) Local Run Manager FASTQ workflow.
NextSeq 1000/2000 folder sizes include local analysis output, which can add another 25-120+ GB depending on workflow.
NovaSeq 6000 folders do not include FASTQ files as part of the total output folder size.
Illumina recommends storing the output folder for iSeq 100, NextSeq 500/550, and NextSeq 1000/2000 runs on a network location. NovaSeq 6000 and NovaSeq X Series Control Software requires the output folder be stored on a network location.
iSeq 100
Read Length
Output Folder Size in GB
2 x 150
1.5 - 2.5
MiniSeq
Mid Output
Read Length
Output Folder Size in GB
2 x 150
2.3 - 2.6
High Output
Read Length
Output Folder Size in GB
2 x 150
7.0 - 8.0
2 x 75
6.0 - 8.0
1 x 75
2.0 - 4.0
Rapid
Read Length
Output Folder Size in GB
1 x 100
2
MiSeq
v3 chemistry
Read Length
Output Folder Size in GB
2 x 300
22 - 26
2 x 75
19 - 22
v2 chemistry
Read Length
Reagent Kit Configuration
Output Folder Size in GB
2 x 250
Standard
22 - 24
2 x 250
Nano
1.6 - 1.8
2 x 150
Standard
16 - 18
2 x 150
Micro
3.0 - 5.0
2 x 150
Nano
0.8 - 1.2
2 x 25
Standard
14 - 16
MiSeq i100 Series
See Knowledge Article Estimating Disk Space Utilization on the MiSeq i100 Series
NextSeq 500/550
Mid Output
Read Length
Output Folder Size in GB
2 x 150
62 - 66
2 x 75
39 - 46
High Output
Read Length
Output Folder Size in GB
2 x 150
160 - 170
2 x 75
92 - 97
1 x 75
50 - 78
NextSeq 1000/2000
P1
Read Length
Output Folder Size in GB
2 x 300
85-95
2 x 150
50 - 55
2 x 101
40 - 45
2 x 75
35 - 40
P2
Read Length
Output Folder Size in GB
2 x 300
140-160
2 x 150
89 - 99
2 x 101
65 - 66
2 x 75
44 - 55
P3
Read Length
Output Folder Size in GB
2 x 150
165 - 170
2 x 101
103 - 126
NovaSeq 6000
SP
Read Length
Output Folder Size in GB
1 x 36
20
2 x 150
185
2 x 250
400
S1
Read Length
Output Folder Size in GB
2 x 150
370
S2
Read Length
Output Folder Size in GB
2 x 150
730
S4
Read Length
Output Folder Size in GB
1 x 36
240
2 x 150
2190
The output folder size in the above tables is reported in Gigabytes (GB).
NovaSeq X/X Plus
Values listed below are PER FLOW cell in Terabytes (TB).
1.5B
Read Length
cBCL Only in TB
BCL Convert (GZIP) in TB
BCL Convert (ORA) in TB
2 x 50
0.054 - 0.091
0.132 - 0.221
0.086 - 0.143
2 x 100
0.106 - 0.181
0.265 - 0.442
0.173 - 0.286
2 x 150
0.163 - 0.272
0.397- 0.662
0.259 - 0.429
10B
Read Length
cBCL Only in TB
BCL Convert (GZIP) in TB
BCL Convert (ORA) in TB
2 x 50
0.34 - 0.57
0.83 - 1.38
0.54 - 0.89
2 x 100
0.68 - 1.13
1.65 - 2.76
1.08 - 1.79
2 x 150
1.02 - 1.70
2.48 - 4.14
1.62 - 2.68
25B
Read Length
cBCL Only in TB
BCL Convert (GZIP) in TB
BCL Convert (ORA) in TB
2 x 50
0.85 - 1.42
2.07 - 3.45
1.35 - 2.23
2 x 100
1.70 - 2.83
4.13 - 6.90
2.70 - 4.47
2 x 150
2.55 - 4.25
6.200 - 10.35
4.05 - 6.70
DRAGEN Analysis files can ADD the following additional data in terabytes (TB) to the expected Output folder size based on Analysis options selected. Approximate file sizes are based on a single flow cell, 2x150 cycle sequencing run.
Flow Cell Type
BAM (TB)
CRAM (TB)
GVCF+VCF (TB)
1.5B
0.4
0.1
<0.1
10B
2.5
1.0
0.2
25B
6.3
2.5
0.5
For any feedback or questions regarding this article (Illumina Knowledge Article #1508), contact Illumina Technical Support techsupport@illumina.com.
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