Best practices for maintaining and cleaning Illumina sequencing system wash cartridges
Illumina NGS (Next Generation Sequencing) platforms require a post-run wash and/or maintenance wash to be performed with regularity (refer to the appropriate instrument guide for details). To avoid contaminating the fluidics lines during these washes, Illumina recommends following these best practices.
- 1.After removing the wash cartridge from the instrument, properly discard any remaining wash solution (eg, do not reuse the Tween wash solution).
- 2.Thoroughly rinse each position of the wash cartridge with lab-grade warm water.
- 3.Place the wash cartridge upside down with some space to allow air flow and proper drying.
- 4.Inspect the wash cartridge wells regularly for contamination (refer to Fig. 1, black or pink discoloration may be noted).
Figure 1. Black ring at bottom of wash cartridge well.
- 1.Prepare a 1–3% lab-grade bleach solution to fill the wells of the tray.
- 2.Use a brush to remove the mold from the wells.
- 3.IMPORTANT: Thoroughly rinse the wells (at least three times) with DI or lab-grade water (residual bleach can cause clustering issues on subsequent runs).
- 4.Place cartridge upside down with some space to allow air flow and proper drying.
- 5.Repeat as necessary if mold is still present.
- 6.If it is not possible to remove all the mold, contact Illumina Technical Support to discuss replacing the wash cartridges.
For step-by-step guidance, refer to this .
For any feedback or questions regarding this article (Illumina Knowledge Article #5283), contact Illumina Technical Support [email protected].